Gene description for Kars
Gene name lysyl-tRNA synthetase
Gene symbol Kars
Other names/aliases AA589550
AL024334
AL033315
AL033367
D8Ertd698e
D8Wsu108e
LysRS
mKIAA0070
Species Mus musculus
 Database cross references - Kars
ExoCarta ExoCarta_85305
Vesiclepedia VP_85305
Entrez Gene 85305
UniProt Q99MN1  
 Kars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kars
Molecular Function
    tRNA binding GO:0000049 IBA
    tRNA binding GO:0000049 ISO
    nucleic acid binding GO:0003676 IEA
    ATP:ADP adenylyltransferase activity GO:0003877 IBA
    ATP:ADP adenylyltransferase activity GO:0003877 ISO
    ATP:ADP adenylyltransferase activity GO:0003877 ISS
    lysine-tRNA ligase activity GO:0004824 IBA
    lysine-tRNA ligase activity GO:0004824 ISO
    lysine-tRNA ligase activity GO:0004824 ISS
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    amino acid binding GO:0016597 ISO
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    scaffold protein binding GO:0097110 IPI
Biological Process
    basophil activation involved in immune response GO:0002276 IBA
    basophil activation involved in immune response GO:0002276 ISO
    positive regulation of inflammatory response to antigenic stimulus GO:0002863 ISO
    lysyl-tRNA aminoacylation GO:0006430 IBA
    lysyl-tRNA aminoacylation GO:0006430 ISO
    lysyl-tRNA aminoacylation GO:0006430 ISS
    response to X-ray GO:0010165 ISO
    diadenosine tetraphosphate biosynthetic process GO:0015966 IBA
    diadenosine tetraphosphate biosynthetic process GO:0015966 ISO
    diadenosine tetraphosphate biosynthetic process GO:0015966 ISS
    positive regulation of macrophage activation GO:0043032 IBA
    positive regulation of macrophage activation GO:0043032 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    ERK1 and ERK2 cascade GO:0070371 ISO
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IBA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 IDA
    aminoacyl-tRNA synthetase multienzyme complex GO:0017101 ISO
 Experiment description of studies that identified Kars in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mettl21e  
Affinity Capture-MS Mus musculus
2 Mars 216443
Co-fractionation Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Lmo2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Hars2  
Co-fractionation Mus musculus
7 Rars 104458
Co-fractionation Mus musculus
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