Gene description for Tkt
Gene name transketolase
Gene symbol Tkt
Other names/aliases p68
Species Mus musculus
 Database cross references - Tkt
ExoCarta ExoCarta_21881
Vesiclepedia VP_21881
Entrez Gene 21881
UniProt P40142  
 Tkt identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Oligodendrocytes 21136642    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Tkt
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    transketolase activity GO:0004802 IBA
    transketolase activity GO:0004802 IDA
    transketolase activity GO:0004802 ISO
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    carbohydrate binding GO:0030246 ISO
    thiamine pyrophosphate binding GO:0030976 IBA
    thiamine pyrophosphate binding GO:0030976 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    monosaccharide binding GO:0048029 ISO
Biological Process
    pentose-phosphate shunt GO:0006098 IDA
    pentose-phosphate shunt GO:0006098 ISO
    pentose-phosphate shunt, non-oxidative branch GO:0009052 IBA
    pentose-phosphate shunt, non-oxidative branch GO:0009052 IMP
    pentose-phosphate shunt, non-oxidative branch GO:0009052 ISO
    regulation of growth GO:0040008 IMP
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 IEA
    glyceraldehyde-3-phosphate biosynthetic process GO:0046166 ISO
    xylulose 5-phosphate biosynthetic process GO:1901159 IDA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    peroxisome GO:0005777 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 ISO
    cytosol GO:0005829 IDA
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    myelin sheath GO:0043209 HDA
 Experiment description of studies that identified Tkt in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Tkt
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
2 Rpl32 19951
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Pygl 110095
Co-fractionation Mus musculus
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Kcnma1  
Affinity Capture-MS Mus musculus
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