Gene description for Pygl
Gene name liver glycogen phosphorylase
Gene symbol Pygl
Other names/aliases -
Species Mus musculus
 Database cross references - Pygl
ExoCarta ExoCarta_110095
Vesiclepedia VP_110095
Entrez Gene 110095
UniProt Q9ET01  
 Pygl identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pygl
Molecular Function
    purine nucleobase binding GO:0002060 IEA
    purine nucleobase binding GO:0002060 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    D-glucose binding GO:0005536 IEA
    D-glucose binding GO:0005536 ISO
    glycogen phosphorylase activity GO:0008184 IBA
    glycogen phosphorylase activity GO:0008184 IDA
    glycogen phosphorylase activity GO:0008184 ISO
    AMP binding GO:0016208 IEA
    AMP binding GO:0016208 ISO
    vitamin binding GO:0019842 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    pyridoxal phosphate binding GO:0030170 IEA
    carbohydrate binding GO:0030246 ISO
    maltodextrin phosphorylase activity GO:0031220 IEA
    bile acid binding GO:0032052 IEA
    bile acid binding GO:0032052 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    glycogen metabolic process GO:0005977 ISO
    glycogen catabolic process GO:0005980 IBA
    glycogen catabolic process GO:0005980 IMP
    glycogen catabolic process GO:0005980 ISO
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 IEA
    5-phosphoribose 1-diphosphate biosynthetic process GO:0006015 ISO
    response to bacterium GO:0009617 IEP
    glucose homeostasis GO:0042593 IEA
    glucose homeostasis GO:0042593 ISO
    necroptotic process GO:0070266 IGI
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
 Experiment description of studies that identified Pygl in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pygl
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Polh  
Co-fractionation Mus musculus
2 Nphp4  
Affinity Capture-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Chordc1  
Co-fractionation Mus musculus
5 Ankmy2  
Co-fractionation Mus musculus
6 Egln1 112405
Co-fractionation Mus musculus
7 Tkt 21881
Co-fractionation Mus musculus
8 Kcna3  
Affinity Capture-MS Mus musculus
9 Pygb 110078
Co-fractionation Mus musculus
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