Gene description for Pygb
Gene name brain glycogen phosphorylase
Gene symbol Pygb
Other names/aliases -
Species Mus musculus
 Database cross references - Pygb
ExoCarta ExoCarta_110078
Vesiclepedia VP_110078
Entrez Gene 110078
UniProt Q8CI94  
 Pygb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pygb
Molecular Function
    glycogen phosphorylase activity GO:0008184 IBA
    glycogen phosphorylase activity GO:0008184 IDA
    glycogen phosphorylase activity GO:0008184 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    pyridoxal phosphate binding GO:0030170 IEA
    carbohydrate binding GO:0030246 ISO
    maltodextrin phosphorylase activity GO:0031220 IEA
    identical protein binding GO:0042802 ISO
    organic cyclic compound binding GO:0097159 ISO
Biological Process
    glycogen metabolic process GO:0005977 ISO
    glycogen catabolic process GO:0005980 IBA
    glycogen catabolic process GO:0005980 IDA
    glycogen catabolic process GO:0005980 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    axon GO:0030424 ISO
 Experiment description of studies that identified Pygb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pygb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Actn1 109711
Co-fractionation Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Uba6 231380
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Pygl 110095
Co-fractionation Mus musculus
6 Mapt  
Affinity Capture-MS Mus musculus
7 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
8 HSD17B10 3028
Affinity Capture-MS Homo sapiens
9 Wdr61  
Co-fractionation Mus musculus
10 Xpnpep1 170750
Co-fractionation Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
12 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
13 Pygb 110078
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
View the network image/svg+xml
 Pathways in which Pygb is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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