Gene description for CYB561A3
Gene name cytochrome b561 family, member A3
Gene symbol CYB561A3
Other names/aliases CYBASC3
LCYTB
Species Homo sapiens
 Database cross references - CYB561A3
ExoCarta ExoCarta_220002
Vesiclepedia VP_220002
Entrez Gene 220002
HGNC 23014
UniProt Q8NBI2  
 CYB561A3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CYB561A3
Molecular Function
    protein binding GO:0005515 IPI
    oxidoreductase activity GO:0016491 IBA
    metal ion binding GO:0046872 IEA
    transmembrane ascorbate ferrireductase activity GO:0140571 ISS
Biological Process
    intracellular iron ion homeostasis GO:0006879 ISS
    transmembrane transport GO:0055085 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 ISS
    late endosome membrane GO:0031902 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified CYB561A3 in sEVs
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CYB561A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TM4SF18  
Two-hybrid Homo sapiens
2 KLRC1  
Two-hybrid Homo sapiens
3 EHHADH 1962
Two-hybrid Homo sapiens
4 GPR152  
Two-hybrid Homo sapiens
5 CD40 958
Two-hybrid Homo sapiens
6 GPX8 493869
Two-hybrid Homo sapiens
7 TMEM31  
Two-hybrid Homo sapiens
8 HERPUD2  
Two-hybrid Homo sapiens
9 LAPTM4B  
Two-hybrid Homo sapiens
10 AGPAT5 55326
Two-hybrid Homo sapiens
11 RNF185  
Two-hybrid Homo sapiens
12 GPR37L1  
Two-hybrid Homo sapiens
13 FFAR2  
Two-hybrid Homo sapiens
14 RNF5  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
15 GPR101  
Two-hybrid Homo sapiens
16 CLDN5 7122
Two-hybrid Homo sapiens
17 CD79A  
Two-hybrid Homo sapiens
18 KIAA1644  
Two-hybrid Homo sapiens
19 CLDN7 1366
Two-hybrid Homo sapiens
20 DDX58 23586
Affinity Capture-RNA Homo sapiens
21 AOC1 26
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which CYB561A3 is involved
No pathways found





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