Gene description for AOC1
Gene name amine oxidase, copper containing 1
Gene symbol AOC1
Other names/aliases ABP
ABP1
DAO
DAO1
KAO
Species Homo sapiens
 Database cross references - AOC1
ExoCarta ExoCarta_26
Vesiclepedia VP_26
Entrez Gene 26
HGNC 80
MIM 104610
UniProt P19801  
 AOC1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Urine 21082674    
 Gene ontology annotations for AOC1
Molecular Function
    copper ion binding GO:0005507 IBA
    copper ion binding GO:0005507 IDA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    primary methylamine oxidase activity GO:0008131 IBA
    primary methylamine oxidase activity GO:0008131 IDA
    heparin binding GO:0008201 IDA
    protein homodimerization activity GO:0042803 IPI
    quinone binding GO:0048038 IEA
    putrescine oxidase activity GO:0050232 IDA
    diamine oxidase activity GO:0052597 IBA
    diamine oxidase activity GO:0052597 IDA
    histamine oxidase activity GO:0052598 IDA
Biological Process
    putrescine metabolic process GO:0009445 IBA
    putrescine metabolic process GO:0009445 IMP
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    peroxisome GO:0005777 NAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    bicellular tight junction GO:0005923 ISS
    specific granule lumen GO:0035580 TAS
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified AOC1 in sEVs
1
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 102
MISEV standards
✔
IEM
Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21082674    
Organism Homo sapiens
Experiment description "Comprehensive analysis of low-abundance proteins in human urinary exosomes using peptide ligand library technology, peptide OFFGEL fractionation and nanoHPLC-chip-MS/MS."
Authors "Zhang Y, Li Y, Qiu F, Qiu Z."
Journal name ELEC
Publication year 2010
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Immunoelectron Microscopy
 Protein-protein interactions for AOC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CYB561A3 220002
Two-hybrid Homo sapiens
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