Gene description for HIST1H2AA
Gene name histone cluster 1, H2aa
Gene symbol HIST1H2AA
Other names/aliases H2AA
H2AFR
bA317E16.2
Species Homo sapiens
 Database cross references - HIST1H2AA
ExoCarta ExoCarta_221613
Vesiclepedia VP_221613
Entrez Gene 221613
HGNC 18729
MIM 613499
UniProt Q96QV6  
 HIST1H2AA identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HIST1H2AA
Molecular Function
    DNA binding GO:0003677 IEA
    structural constituent of chromatin GO:0030527 IBA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    heterochromatin formation GO:0031507 IBA
Subcellular Localization
    chromosome, telomeric region GO:0000781 HDA
    nucleosome GO:0000786 IBA
    female germ cell nucleus GO:0001674 IEA
    nucleus GO:0005634 HDA
    nucleus GO:0005634 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified HIST1H2AA in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HIST1H2AA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Co-fractionation Homo sapiens
2 HIST2H2BE 8349
Cross-Linking-MS (XL-MS) Homo sapiens
3 H2AFJ 55766
Cross-Linking-MS (XL-MS) Homo sapiens
4 HNRNPD 3184
Affinity Capture-MS Homo sapiens
5 TFCP2 7024
Affinity Capture-MS Homo sapiens
6 RARA 5914
Affinity Capture-MS Homo sapiens
7 HDAC2 3066
Reconstituted Complex Homo sapiens
8 RPS4X 6191
Co-fractionation Homo sapiens
9 FANCD2  
Affinity Capture-MS Homo sapiens
10 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
11 HIST1H4A 8359
Cross-Linking-MS (XL-MS) Homo sapiens
12 BKRF4  
Affinity Capture-MS
13 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
14 MCM5 4174
Affinity Capture-MS Homo sapiens
15 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
16 ACTC1 70
Proximity Label-MS Homo sapiens
17 HIST1H2BJ 8970
Cross-Linking-MS (XL-MS) Homo sapiens
18 HIST2H2AB 317772
Cross-Linking-MS (XL-MS) Homo sapiens
19 KIF20A 10112
Affinity Capture-MS Homo sapiens
20 HIST1H2BB 3018
Cross-Linking-MS (XL-MS) Homo sapiens
Co-fractionation Homo sapiens
21 EGFR 1956
Affinity Capture-MS Homo sapiens
22 HDAC1 3065
Reconstituted Complex Homo sapiens
23 PARK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 EIF4A3 9775
Affinity Capture-MS Homo sapiens
25 DOT1L 84444
Affinity Capture-MS Homo sapiens
26 FN1 2335
Affinity Capture-MS Homo sapiens
27 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
28 KIAA1429 25962
Affinity Capture-MS Homo sapiens
29 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
30 HIST1H2BD 3017
Cross-Linking-MS (XL-MS) Homo sapiens
31 HIST2H2BF 440689
Cross-Linking-MS (XL-MS) Homo sapiens
32 HDAC3 8841
Reconstituted Complex Homo sapiens
33 HIST3H2BB 128312
Cross-Linking-MS (XL-MS) Homo sapiens
34 YAP1 10413
Affinity Capture-MS Homo sapiens
35 HIST1H2BA 255626
Co-fractionation Homo sapiens
36 HIST3H2A 92815
Cross-Linking-MS (XL-MS) Homo sapiens
37 MCM2 4171
Affinity Capture-MS Homo sapiens
38 RPS14 6208
Co-fractionation Homo sapiens
39 RAD18  
Affinity Capture-Western Homo sapiens
40 ANP32B 10541
Reconstituted Complex Homo sapiens
41 GRSF1 2926
Proximity Label-MS Homo sapiens
42 SERBP1 26135
Affinity Capture-MS Homo sapiens
43 NPM1 4869
Affinity Capture-MS Homo sapiens
44 MAGOH 4116
Affinity Capture-MS Homo sapiens
45 OBSL1 23363
Affinity Capture-MS Homo sapiens
46 PRKACB 5567
Affinity Capture-MS Homo sapiens
47 HIST2H2AC 8338
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml



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