Gene description for FCN2
Gene name ficolin (collagen/fibrinogen domain containing lectin) 2
Gene symbol FCN2
Other names/aliases EBP-37
FCNL
P35
ficolin-2
Species Homo sapiens
 Database cross references - FCN2
ExoCarta ExoCarta_2220
Vesiclepedia VP_2220
Entrez Gene 2220
HGNC 3624
MIM 601624
UniProt Q15485  
 FCN2 identified in sEVs derived from the following tissue/cell type
Plasma 19028452    
Urine 19056867    
 Gene ontology annotations for FCN2
Molecular Function
    antigen binding GO:0003823 IBA
    antigen binding GO:0003823 IDA
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    proteoglycan binding GO:0043394 IDA
    metal ion binding GO:0046872 IEA
    calcium-dependent protein binding GO:0048306 IPI
    carbohydrate derivative binding GO:0097367 IBA
    mannan binding GO:2001065 IDA
Biological Process
    complement activation, lectin pathway GO:0001867 IBA
    complement activation, lectin pathway GO:0001867 IDA
    cell surface pattern recognition receptor signaling pathway GO:0002752 NAS
    proteolysis GO:0006508 IDA
    opsonization GO:0008228 IDA
    recognition of apoptotic cell GO:0043654 IDA
    defense response to Gram-negative bacterium GO:0050829 IDA
    defense response to Gram-positive bacterium GO:0050830 IDA
    positive regulation of opsonization GO:1903028 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 NAS
    external side of plasma membrane GO:0009897 NAS
    collagen-containing extracellular matrix GO:0062023 IBA
    extracellular exosome GO:0070062 HDA
    blood microparticle GO:0072562 HDA
    serine-type endopeptidase complex GO:1905370 IDA
 Experiment description of studies that identified FCN2 in sEVs
1
Experiment ID 44
MISEV standards
IEM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors "Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ"
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for FCN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MASP1 5648
Reconstituted Complex Homo sapiens
2 FCN1 2219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MASP2 10747
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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