Experiment description of studies that identified NKAPL in sEVs

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for NKAPL

Protein Interactor ExoCarta ID Identification method PubMed Species 1 PRC1 9055 Affinity Capture-MS Homo sapiens 2 ZNF587   Two-hybrid Homo sapiens 3 TNFRSF10D 8793 Affinity Capture-MS Homo sapiens 4 DDX41 51428 Two-hybrid Homo sapiens 5 MECP2 4204 Affinity Capture-MS Homo sapiens 6 C9orf72   Affinity Capture-MS Homo sapiens 7 BRCA1 672 Two-hybrid Homo sapiens 8 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 9 ARL6IP4   Two-hybrid Homo sapiens 10 CUL2 8453 Affinity Capture-MS Homo sapiens 11 SQSTM1 8878 Proximity Label-MS Homo sapiens 12 SREK1IP1   Two-hybrid Homo sapiens 13 ZNF417   Two-hybrid Homo sapiens 14 CLK3   Two-hybrid Homo sapiens 15 CSNK2A2 1459 Affinity Capture-MS Homo sapiens 16 NKAP 79576 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 C11orf57   Two-hybrid Homo sapiens View the network

Pathways in which NKAPL is involved

No pathways found