Gene description for Eif3l
Gene name eukaryotic translation initiation factor 3, subunit L
Gene symbol Eif3l
Other names/aliases 0610011H21Rik
D15N1e
Eif3eip
Eif3ip
Eif3s6ip
HSP-66Y
PAF67
Species Mus musculus
 Database cross references - Eif3l
ExoCarta ExoCarta_223691
Vesiclepedia VP_223691
Entrez Gene 223691
UniProt Q8QZY1  
 Eif3l identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eif3l
Molecular Function
    translation initiation factor activity GO:0003743 IDA
    translation initiation factor activity GO:0003743 ISO
    protein binding GO:0005515 IPI
Biological Process
    formation of cytoplasmic translation initiation complex GO:0001732 IEA
    translational initiation GO:0006413 IBA
    translational initiation GO:0006413 IDA
    translational initiation GO:0006413 ISO
    viral translational termination-reinitiation GO:0075525 IEA
    viral translational termination-reinitiation GO:0075525 ISO
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 IBA
    eukaryotic translation initiation factor 3 complex GO:0005852 IDA
    eukaryotic translation initiation factor 3 complex GO:0005852 ISO
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
 Experiment description of studies that identified Eif3l in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eif3l
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Fzr1  
Affinity Capture-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 RRN3  
Affinity Capture-Western Homo sapiens
6 Chaf1a  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
8 Ubtf 21429
Affinity Capture-Western Mus musculus
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