Gene description for WDR43
Gene name WD repeat domain 43
Gene symbol WDR43
Other names/aliases NET12
UTP5
Species Homo sapiens
 Database cross references - WDR43
ExoCarta ExoCarta_23160
Vesiclepedia VP_23160
Entrez Gene 23160
HGNC 28945
MIM 616195
UniProt Q15061  
 WDR43 identified in sEVs derived from the following tissue/cell type
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for WDR43
Molecular Function
    RNA polymerase II complex binding GO:0000993 ISS
    transcription elongation factor activity GO:0003711 ISS
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IBA
    regulation of transcription elongation by RNA polymerase II GO:0034243 ISS
    ribosomal small subunit biogenesis GO:0042274 IDA
    positive regulation of transcription by RNA polymerase I GO:0045943 IMP
    regulation of stem cell population maintenance GO:2000036 ISS
    positive regulation of rRNA processing GO:2000234 IMP
Subcellular Localization
    chromatin GO:0000785 ISS
    fibrillar center GO:0001650 IDA
    nucleoplasm GO:0005654 ISS
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
    small-subunit processome GO:0032040 IDA
 Experiment description of studies that identified WDR43 in sEVs
1
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
4
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WDR43
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NOL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 CD3EAP  
Proximity Label-MS Homo sapiens
3 WDR46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NPM3 10360
Affinity Capture-MS Homo sapiens
5 ANLN 54443
Affinity Capture-MS Homo sapiens
6 KLHL20  
Affinity Capture-MS Homo sapiens
7 FGFBP1 9982
Affinity Capture-MS Homo sapiens
8 USP36  
Affinity Capture-MS Homo sapiens
9 FASN 2194
Positive Genetic Homo sapiens
10 MPHOSPH6 10200
Affinity Capture-MS Homo sapiens
11 PARP1 142
Proximity Label-MS Homo sapiens
12 MAD2L1 4085
Affinity Capture-MS Homo sapiens
13 RPS24 6229
Proximity Label-MS Homo sapiens
14 DIS3 22894
Affinity Capture-MS Homo sapiens
15 KIF14 9928
Affinity Capture-MS Homo sapiens
16 CHCHD1  
Proximity Label-MS Homo sapiens
17 CIAO1 9391
Affinity Capture-MS Homo sapiens
18 XPO1 7514
Affinity Capture-MS Homo sapiens
19 LIN28A  
Affinity Capture-MS Homo sapiens
20 ECT2 1894
Affinity Capture-MS Homo sapiens
21 RIT1 6016
Negative Genetic Homo sapiens
22 SIRT7  
Affinity Capture-MS Homo sapiens
23 FGF12  
Affinity Capture-MS Homo sapiens
24 APEX1 328
Proximity Label-MS Homo sapiens
25 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
26 NOP56 10528
Proximity Label-MS Homo sapiens
27 CD6 923
Affinity Capture-MS Homo sapiens
28 CERK 64781
Affinity Capture-MS Homo sapiens
29 MAPK3 5595
Affinity Capture-MS Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 NUP50 10762
Proximity Label-MS Homo sapiens
32 EGFR 1956
Negative Genetic Homo sapiens
33 NAP1L1 4673
Co-fractionation Homo sapiens
34 UTP15 84135
Affinity Capture-MS Homo sapiens
35 RRP9 9136
Affinity Capture-MS Homo sapiens
36 DDX23 9416
Proximity Label-MS Homo sapiens
37 SRSF3 6428
Affinity Capture-MS Homo sapiens
38 NIFK 84365
Proximity Label-MS Homo sapiens
39 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
40 NXF1 10482
Affinity Capture-RNA Homo sapiens
41 NOL11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 UFL1 23376
Affinity Capture-MS Homo sapiens
43 WDR75  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CBX3 11335
Proximity Label-MS Homo sapiens
45 KIF20A 10112
Affinity Capture-MS Homo sapiens
46 RBM4 5936
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 UBR5 51366
Co-fractionation Homo sapiens
48 CIRH1A 84916
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 NCL 4691
Co-fractionation Homo sapiens
50 EXOSC2 23404
Affinity Capture-MS Homo sapiens
51 PRC1 9055
Affinity Capture-MS Homo sapiens
52 VCP 7415
Affinity Capture-MS Homo sapiens
53 HECTD1 25831
Affinity Capture-MS Homo sapiens
54 KIF23 9493
Affinity Capture-MS Homo sapiens
55 RPL31 6160
Proximity Label-MS Homo sapiens
56 EXOSC3 51010
Affinity Capture-MS Homo sapiens
57 ING5  
Affinity Capture-MS Homo sapiens
58 IFI16 3428
Affinity Capture-MS Homo sapiens
59 NFKBIL1  
Affinity Capture-MS Homo sapiens
60 RPS6 6194
Proximity Label-MS Homo sapiens
61 EXOSC5 56915
Affinity Capture-MS Homo sapiens
62 GRSF1 2926
Proximity Label-MS Homo sapiens
63 MYCN  
Affinity Capture-MS Homo sapiens
64 C1D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CUL7 9820
Affinity Capture-MS Homo sapiens
66 DDRGK1 65992
Affinity Capture-MS Homo sapiens
67 RNF2  
Affinity Capture-MS Homo sapiens
68 UTP18  
Affinity Capture-MS Homo sapiens
69 FBL 2091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 POLR1E  
Proximity Label-MS Homo sapiens
71 OBSL1 23363
Affinity Capture-MS Homo sapiens
72 HIST1H1T 3010
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 RBM4B  
Affinity Capture-MS Homo sapiens
75 KRAS 3845
Negative Genetic Homo sapiens
76 NPM1 4869
Affinity Capture-MS Homo sapiens
77 Ccdc9  
Affinity Capture-MS Mus musculus
78 Nhp2l1 20826
Affinity Capture-MS Mus musculus
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