Gene description for CIAO1
Gene name cytosolic iron-sulfur assembly component 1
Gene symbol CIAO1
Other names/aliases CIA1
WDR39
Species Homo sapiens
 Database cross references - CIAO1
ExoCarta ExoCarta_9391
Vesiclepedia VP_9391
Entrez Gene 9391
HGNC 14280
MIM 604333
UniProt O76071  
 CIAO1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for CIAO1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 TAS
    chromosome segregation GO:0007059 IEA
    positive regulation of cell population proliferation GO:0008284 TAS
    iron-sulfur cluster assembly GO:0016226 IBA
    iron-sulfur cluster assembly GO:0016226 IGI
    iron-sulfur cluster assembly GO:0016226 IMP
    protein maturation by iron-sulfur cluster transfer GO:0097428 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    MMXD complex GO:0071817 IDA
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IBA
    cytosolic [4Fe-4S] assembly targeting complex GO:0097361 IDA
 Experiment description of studies that identified CIAO1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CIAO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 PRIM2 5558
Affinity Capture-MS Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 DPP9 91039
Affinity Capture-MS Homo sapiens
6 Mms19  
Affinity Capture-MS Mus musculus
7 ELP6 54859
Affinity Capture-MS Homo sapiens
8 TSC2 7249
Affinity Capture-MS Homo sapiens
9 HCST  
Affinity Capture-MS Homo sapiens
10 RBM15B  
Affinity Capture-MS Homo sapiens
11 PPIL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TRIM66  
Affinity Capture-MS Homo sapiens
13 EPB41L5 57669
Affinity Capture-MS Homo sapiens
14 SLC2A1 6513
Affinity Capture-MS Homo sapiens
15 C9orf78 51759
Affinity Capture-MS Homo sapiens
16 TLE3  
Affinity Capture-MS Homo sapiens
17 GAS8  
Affinity Capture-MS Homo sapiens
18 MRE11A 4361
Affinity Capture-MS Homo sapiens
19 PPAT 5471
Affinity Capture-MS Homo sapiens
20 SLC30A4 7782
Affinity Capture-MS Homo sapiens
21 APP 351
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
22 DNAJB6 10049
Affinity Capture-MS Homo sapiens
23 CTU2 348180
Affinity Capture-MS Homo sapiens
24 UGDH 7358
Affinity Capture-MS Homo sapiens
25 PARK2  
Affinity Capture-MS Homo sapiens
26 TBL2 26608
Affinity Capture-MS Homo sapiens
27 HOXA5  
Affinity Capture-MS Homo sapiens
28 WT1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
29 NUP35 129401
Affinity Capture-MS Homo sapiens
30 DUSP11  
Affinity Capture-MS Homo sapiens
31 PRIM1  
Affinity Capture-MS Homo sapiens
32 LTA4H 4048
Affinity Capture-MS Homo sapiens
33 CPLX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 FMNL2 114793
Affinity Capture-MS Homo sapiens
35 ABCE1 6059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 ELP4  
Affinity Capture-MS Homo sapiens
37 NONO 4841
Affinity Capture-MS Homo sapiens
38 CSNK1D 1453
Affinity Capture-MS Homo sapiens
39 NPAS1  
Affinity Capture-MS Homo sapiens
40 WDR5 11091
Affinity Capture-Western Homo sapiens
41 MAK16  
Affinity Capture-MS Homo sapiens
42 CTAGE5 4253
Affinity Capture-MS Homo sapiens
43 FBXL5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
44 CCT2 10576
Affinity Capture-MS Homo sapiens
45 UCKL1 54963
Affinity Capture-MS Homo sapiens
46 CD70 970
Affinity Capture-MS Homo sapiens
47 DPP8 54878
Affinity Capture-MS Homo sapiens
48 POLD1 5424
Affinity Capture-MS Homo sapiens
49 TCP10L  
Two-hybrid Homo sapiens
50 SENP2 59343
Affinity Capture-MS Homo sapiens
51 RGS2  
Two-hybrid Homo sapiens
52 TOP3B 8940
Affinity Capture-MS Homo sapiens
53 USP11 8237
Affinity Capture-MS Homo sapiens
54 ERCC2 2068
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
55 WBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 MEPCE 56257
Affinity Capture-MS Homo sapiens
57 PHAX  
Affinity Capture-MS Homo sapiens
58 CCAR2 57805
Affinity Capture-MS Homo sapiens
59 FAM96B 51647
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
60 ILVBL 10994
Affinity Capture-MS Homo sapiens
61 POLA2  
Affinity Capture-MS Homo sapiens
62 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 WDR61 80349
Affinity Capture-MS Homo sapiens
64 COMTD1 118881
Affinity Capture-MS Homo sapiens
65 CDKAL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RFESD  
Affinity Capture-MS Homo sapiens
67 ERP29 10961
Affinity Capture-MS Homo sapiens
68 MEOX2  
Two-hybrid Homo sapiens
69 WDR43 23160
Affinity Capture-MS Homo sapiens
70 MRPL3  
Affinity Capture-MS Homo sapiens
71 IDH3G 3421
Affinity Capture-MS Homo sapiens
72 CTR9 9646
Affinity Capture-MS Homo sapiens
73 POLE  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 CCDC7  
Two-hybrid Homo sapiens
75 AQP12B  
Affinity Capture-MS Homo sapiens
76 MRPL9 65005
Affinity Capture-MS Homo sapiens
77 SOX1 6656
Affinity Capture-MS Homo sapiens
78 MLF2 8079
Affinity Capture-MS Homo sapiens
79 GTPBP4 23560
Affinity Capture-MS Homo sapiens
80 SRPK1 6732
Biochemical Activity Homo sapiens
81 RTEL1 51750
Affinity Capture-MS Homo sapiens
82 GOLGA3 2802
Affinity Capture-MS Homo sapiens
83 YAE1D1  
Affinity Capture-MS Homo sapiens
84 LEO1 123169
Affinity Capture-MS Homo sapiens
85 CDC73  
Affinity Capture-MS Homo sapiens
86 CTU1 90353
Affinity Capture-MS Homo sapiens
87 MRPS11  
Affinity Capture-MS Homo sapiens
88 CCT6A 908
Affinity Capture-MS Homo sapiens
89 UQCRC2 7385
Affinity Capture-MS Homo sapiens
90 MEX3A  
Affinity Capture-RNA Homo sapiens
91 ELP3 55140
Affinity Capture-MS Homo sapiens
92 SPC24 147841
Affinity Capture-MS Homo sapiens
93 CNP 1267
Affinity Capture-MS Homo sapiens
94 SF1 7536
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
95 DCAF13 25879
Affinity Capture-MS Homo sapiens
96 EGFR 1956
Negative Genetic Homo sapiens
97 DDX31  
Affinity Capture-MS Homo sapiens
98 ELP2 55250
Affinity Capture-MS Homo sapiens
99 LARS 51520
Affinity Capture-MS Homo sapiens
100 MYO1C 4641
Affinity Capture-MS Homo sapiens
101 PLEKHA5 54477
Affinity Capture-MS Homo sapiens
102 RGS13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 NSRP1  
Affinity Capture-MS Homo sapiens
104 GNB2 2783
Affinity Capture-MS Homo sapiens
105 INO80B 83444
Affinity Capture-MS Homo sapiens
106 FAM60A  
Affinity Capture-MS Homo sapiens
107 WDR75  
Affinity Capture-MS Homo sapiens
108 UPF3B 65109
Affinity Capture-MS Homo sapiens
109 B3GAT3 26229
Affinity Capture-MS Homo sapiens
110 RPS29 6235
Affinity Capture-MS Homo sapiens
111 NOP16 51491
Affinity Capture-MS Homo sapiens
112 NARFL 64428
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
113 MTM1 4534
Affinity Capture-MS Homo sapiens
114 LANCL2 55915
Affinity Capture-MS Homo sapiens
115 HAUS3  
Two-hybrid Homo sapiens
116 EEPD1 80820
Affinity Capture-MS Homo sapiens
117 ZRANB1 54764
Affinity Capture-MS Homo sapiens
118 MEOX1  
Two-hybrid Homo sapiens
119 PAFAH1B1 5048
Affinity Capture-MS Homo sapiens
120 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
121 FIZ1  
Affinity Capture-MS Homo sapiens
122 CCT8 10694
Affinity Capture-MS Homo sapiens
123 TOE1 114034
Affinity Capture-MS Homo sapiens
124 SHISA6  
Two-hybrid Homo sapiens
125 URI1  
Two-hybrid Homo sapiens
126 PGRMC1 10857
Two-hybrid Homo sapiens
127 IREB2 3658
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
128 MFSD4  
Affinity Capture-MS Homo sapiens
129 MCM5 4174
Affinity Capture-MS Homo sapiens
130 DNA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 UBA52 7311
Affinity Capture-MS Homo sapiens
132 TYW1  
Affinity Capture-MS Homo sapiens
133 DNAAF5 54919
Affinity Capture-MS Homo sapiens
134 CHD1L 9557
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
135 TSC1 7248
Affinity Capture-MS Homo sapiens
136 CLEC4C  
Affinity Capture-MS Homo sapiens
137 FMNL3 91010
Affinity Capture-MS Homo sapiens
138 CDK1 983
Affinity Capture-MS Homo sapiens
139 CCT7 10574
Affinity Capture-MS Homo sapiens
140 AP1M1 8907
Affinity Capture-MS Homo sapiens
141 PAF1 54623
Affinity Capture-MS Homo sapiens
142 MMS19 64210
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
143 KDELR2 11014
Affinity Capture-MS Homo sapiens
144 CDK9 1025
Affinity Capture-MS Homo sapiens
145 BRD1 23774
Affinity Capture-MS Homo sapiens
146 POLA1  
Affinity Capture-MS Homo sapiens
147 GLRX3 10539
Affinity Capture-MS Homo sapiens
148 ELP5 23587
Affinity Capture-MS Homo sapiens
149 KRAS 3845
Negative Genetic Homo sapiens
150 FAM96A  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
151 RPL36AL 6166
Affinity Capture-MS Homo sapiens
152 RSAD2  
Affinity Capture-Western Homo sapiens
153 BCL7A  
Co-fractionation Homo sapiens
154 DDX56  
Affinity Capture-MS Homo sapiens
155 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
156 KIAA1644  
Affinity Capture-MS Homo sapiens
157 ACAA2 10449
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CIAO1 is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome
Metabolism IEA Reactome





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