Gene description for DPP9
Gene name dipeptidyl-peptidase 9
Gene symbol DPP9
Other names/aliases DP9
DPLP9
DPRP-2
DPRP2
Species Homo sapiens
 Database cross references - DPP9
ExoCarta ExoCarta_91039
Vesiclepedia VP_91039
Entrez Gene 91039
HGNC 18648
MIM 608258
UniProt Q86TI2  
 DPP9 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
 Gene ontology annotations for DPP9
Molecular Function
    aminopeptidase activity GO:0004177 IEA
    protein binding GO:0005515 IPI
    serine-type peptidase activity GO:0008236 IEA
    dipeptidyl-peptidase activity GO:0008239 IBA
    dipeptidyl-peptidase activity GO:0008239 IDA
    dipeptidyl-peptidase activity GO:0008239 IMP
    identical protein binding GO:0042802 IPI
Biological Process
    proteolysis GO:0006508 IBA
    negative regulation of programmed cell death GO:0043069 IDA
    pyroptotic inflammatory response GO:0070269 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 IDA
    microtubule GO:0005874 IDA
    cell leading edge GO:0031252 IDA
 Experiment description of studies that identified DPP9 in sEVs
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 426
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 427
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 419
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 420
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
12
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
13
Experiment ID 434
MISEV standards
Biophysical techniques
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 435
MISEV standards
Biophysical techniques
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
CANX
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DPP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLLU1OS  
Affinity Capture-MS Homo sapiens
2 GJD4  
Affinity Capture-MS Homo sapiens
3 NPLOC4 55666
Co-fractionation Homo sapiens
4 STAT2 6773
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 STAT5B 6777
Co-fractionation Homo sapiens
6 SPRED2 200734
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FOXF2  
Affinity Capture-MS Homo sapiens
8 DOK3 79930
Affinity Capture-MS Homo sapiens
9 MRFAP1  
Affinity Capture-MS Homo sapiens
10 FAM163B  
Affinity Capture-MS Homo sapiens
11 HRAS 3265
Reconstituted Complex Homo sapiens
12 FAXC  
Affinity Capture-MS Homo sapiens
13 SURF2  
Co-fractionation Homo sapiens
14 MEGF9 1955
Affinity Capture-MS Homo sapiens
15 DCT 1638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 STOX1  
Affinity Capture-MS Homo sapiens
17 ELFN2 114794
Affinity Capture-MS Homo sapiens
18 CARD8  
Affinity Capture-Western Homo sapiens
19 LPP 4026
Co-fractionation Homo sapiens
20 TOM1L1 10040
Co-fractionation Homo sapiens
21 DNAJC7 7266
Proximity Label-MS Homo sapiens
22 TRMT1 55621
Affinity Capture-MS Homo sapiens
23 TIMM50 92609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NIF3L1 60491
Co-fractionation Homo sapiens
25 LPPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 BCL11B  
Affinity Capture-MS Homo sapiens
27 HOXB3 3213
Affinity Capture-MS Homo sapiens
28 CCDC102A 92922
Affinity Capture-MS Homo sapiens
29 ZADH2 284273
Affinity Capture-MS Homo sapiens
30 GAGE8  
Affinity Capture-MS Homo sapiens
31 CADM4 199731
Affinity Capture-MS Homo sapiens
32 ACOT11 26027
Affinity Capture-MS Homo sapiens
33 DPP8 54878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 ENSA 2029
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SHMT2 6472
Affinity Capture-MS Homo sapiens
36 SPDYC  
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 SAE1 10055
Co-fractionation Homo sapiens
39 BRE 9577
Affinity Capture-MS Homo sapiens
40 FAM19A3  
Affinity Capture-MS Homo sapiens
41 CYS1 192668
Affinity Capture-MS Homo sapiens
42 APOL2 23780
Affinity Capture-MS Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 THG1L 54974
Co-fractionation Homo sapiens
45 CSPG5 10675
Affinity Capture-MS Homo sapiens
46 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
47 MTR 4548
Co-fractionation Homo sapiens
48 CLEC5A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 COX5B 1329
Affinity Capture-MS Homo sapiens
50 ZNF664  
Affinity Capture-MS Homo sapiens
51 CIAO1 9391
Affinity Capture-MS Homo sapiens
52 TMCO6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 IGFL1 374918
Affinity Capture-MS Homo sapiens
54 Bach1 12013
Affinity Capture-MS Mus musculus
55 DMTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DUS3L 56931
Co-fractionation Homo sapiens
57 ARHGAP25  
Affinity Capture-MS Homo sapiens
58 MYO6 4646
Co-fractionation Homo sapiens
59 RPA3 6119
Proximity Label-MS Homo sapiens
60 NLRP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
61 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
62 AP2A2 161
Co-fractionation Homo sapiens
63 DLL1  
Affinity Capture-MS Homo sapiens
64 TP53RK 112858
Co-fractionation Homo sapiens
65 GAS2L2  
Affinity Capture-MS Homo sapiens
66 NTSR1  
Affinity Capture-MS Homo sapiens
67 AARS 16
Co-fractionation Homo sapiens
68 EEF2K  
Co-fractionation Homo sapiens
69 ABHD12 26090
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SLC16A8  
Affinity Capture-MS Homo sapiens
71 FAM175B 23172
Affinity Capture-MS Homo sapiens
72 SNAI1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 TP53 7157
Affinity Capture-MS Homo sapiens
74 HSPA8 3312
Affinity Capture-MS Homo sapiens
75 DNAJA2 10294
Proximity Label-MS Homo sapiens
76 ISCA2 122961
Affinity Capture-MS Homo sapiens
77 POLR3H  
Affinity Capture-MS Homo sapiens
78 KEAP1 9817
Affinity Capture-MS Homo sapiens
79 NSUN5P2  
Affinity Capture-MS Homo sapiens
80 ZNF69  
Affinity Capture-MS Homo sapiens
81 TKT 7086
Affinity Capture-MS Homo sapiens
82 LRRC4  
Affinity Capture-MS Homo sapiens
83 GLB1L3  
Affinity Capture-MS Homo sapiens
84 SLC2A7  
Affinity Capture-MS Homo sapiens
85 TXN 7295
Affinity Capture-Western Homo sapiens
86 NEK2  
Affinity Capture-MS Homo sapiens
87 ANKRD10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 HNRNPF 3185
Affinity Capture-MS Homo sapiens
89 RPRD1B 58490
Co-fractionation Homo sapiens
90 NEFL 4747
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 PARD6A  
Affinity Capture-MS Homo sapiens
92 GRIA2  
Affinity Capture-MS Homo sapiens
93 LCP1 3936
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which DPP9 is involved
No pathways found





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