Gene description for THG1L
Gene name tRNA-histidine guanylyltransferase 1-like (S. cerevisiae)
Gene symbol THG1L
Other names/aliases ICF45
IHG-1
Species Homo sapiens
 Database cross references - THG1L
ExoCarta ExoCarta_54974
Vesiclepedia VP_54974
Entrez Gene 54974
HGNC 26053
UniProt Q9NWX6  
 THG1L identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for THG1L
Molecular Function
    tRNA binding GO:0000049 TAS
    magnesium ion binding GO:0000287 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    GTP binding GO:0005525 IDA
    tRNA guanylyltransferase activity GO:0008193 IBA
    tRNA guanylyltransferase activity GO:0008193 IDA
    tRNA guanylyltransferase activity GO:0008193 ISS
    nucleotidyltransferase activity GO:0016779 EXP
    identical protein binding GO:0042802 IPI
Biological Process
    tRNA modification GO:0006400 ISS
    tRNA modification GO:0006400 TAS
    response to oxidative stress GO:0006979 IDA
    tRNA processing GO:0008033 IBA
    tRNA processing GO:0008033 IDA
    mitochondrial fusion GO:0008053 IDA
    protein homotetramerization GO:0051289 IPI
    tRNA 5'-end processing GO:0099116 IEA
    stress-induced mitochondrial fusion GO:1990046 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IEA
    cytosol GO:0005829 TAS
    transferase complex GO:1990234 IDA
 Experiment description of studies that identified THG1L in sEVs
1
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for THG1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MTR 4548
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 CALR 811
Co-fractionation Homo sapiens
4 SH3GLB1 51100
Co-fractionation Homo sapiens
5 PMPCB 9512
Proximity Label-MS Homo sapiens
6 TACO1  
Proximity Label-MS Homo sapiens
7 SDCBP 6386
Two-hybrid Homo sapiens
8 TSFM 10102
Proximity Label-MS Homo sapiens
9 AUH 549
Proximity Label-MS Homo sapiens
10 PLS3 5358
Co-fractionation Homo sapiens
11 MRRF  
Proximity Label-MS Homo sapiens
12 TPD52L2 7165
Co-fractionation Homo sapiens
13 MDH2 4191
Proximity Label-MS Homo sapiens
14 CLPP 8192
Proximity Label-MS Homo sapiens
15 SH3GL1 6455
Co-fractionation Homo sapiens
16 MTIF3  
Proximity Label-MS Homo sapiens
17 CALU 813
Co-fractionation Homo sapiens
18 WDR92 116143
Co-fractionation Homo sapiens
19 NSUN2 54888
Co-fractionation Homo sapiens
20 PACSIN2 11252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C6orf203  
Proximity Label-MS Homo sapiens
22 PLEKHF2 79666
Two-hybrid Homo sapiens
23 ARPC2 10109
Co-fractionation Homo sapiens
24 SH3BP5L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 UBC 7316
Reconstituted Complex Homo sapiens
26 EGFR 1956
Negative Genetic Homo sapiens
27 ELMOD1  
Two-hybrid Homo sapiens
28 EEF2K  
Co-fractionation Homo sapiens
29 C21orf33  
Proximity Label-MS Homo sapiens
30 MTRF1  
Proximity Label-MS Homo sapiens
31 AARSD1 80755
Co-fractionation Homo sapiens
32 TERF1 7013
Two-hybrid Homo sapiens
33 TP53RK 112858
Co-fractionation Homo sapiens
34 TBRG4 9238
Proximity Label-MS Homo sapiens
35 PCCA 5095
Co-fractionation Homo sapiens
36 DPP9 91039
Co-fractionation Homo sapiens
37 PMPCA 23203
Proximity Label-MS Homo sapiens
38 HARS 3035
Co-fractionation Homo sapiens
39 ZNF524  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CHCHD2  
Affinity Capture-MS Homo sapiens
41 ACAD9 28976
Proximity Label-MS Homo sapiens
42 SH3GLB2 56904
Co-fractionation Homo sapiens
43 HINT2 84681
Proximity Label-MS Homo sapiens
44 THG1L 54974
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
46 TPRKB 51002
Co-fractionation Homo sapiens
47 MRPL11 65003
Proximity Label-MS Homo sapiens
48 PRKAR1B  
Co-fractionation Homo sapiens
49 MRPS26 64949
Proximity Label-MS Homo sapiens
50 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
51 RANGAP1 5905
Co-fractionation Homo sapiens
52 ARPC1A 10552
Co-fractionation Homo sapiens
53 WDYHV1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which THG1L is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





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