Gene description for DUS3L
Gene name dihydrouridine synthase 3-like (S. cerevisiae)
Gene symbol DUS3L
Other names/aliases DUS3
Species Homo sapiens
 Database cross references - DUS3L
ExoCarta ExoCarta_56931
Vesiclepedia VP_56931
Entrez Gene 56931
HGNC 26920
UniProt Q96G46  
 DUS3L identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for DUS3L
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    tRNA dihydrouridine synthase activity GO:0017150 IBA
    metal ion binding GO:0046872 IEA
    flavin adenine dinucleotide binding GO:0050660 IEA
    tRNA-dihydrouridine47 synthase activity GO:0102265 IDA
    mRNA dihydrouridine synthase activity GO:0106414 IDA
Biological Process
    tRNA dihydrouridine synthesis GO:0002943 IDA
    tRNA dihydrouridine synthesis GO:0002943 IMP
    mRNA processing GO:0006397 IEA
    regulation of translation GO:0006417 IDA
 Experiment description of studies that identified DUS3L in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DUS3L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 EIF1AD 84285
Affinity Capture-MS Homo sapiens
3 GTF2F1 2962
Co-fractionation Homo sapiens
4 GPIHBP1  
Affinity Capture-MS Homo sapiens
5 SEC23A 10484
Co-fractionation Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 ACAT1 38
Co-fractionation Homo sapiens
8 DPP9 91039
Co-fractionation Homo sapiens
9 SARS 6301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
10 AVIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ECT2 1894
Affinity Capture-MS Homo sapiens
12 NCAPH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CALU 813
Co-fractionation Homo sapiens
14 ISCA1  
Affinity Capture-MS Homo sapiens
15 CUL3 8452
Affinity Capture-MS Homo sapiens
16 SSB 6741
Affinity Capture-MS Homo sapiens
17 TRMT6 51605
Co-fractionation Homo sapiens
18 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
19 EIF2A 83939
Co-fractionation Homo sapiens
20 SNF8 11267
Co-fractionation Homo sapiens
21 MUS81  
Synthetic Growth Defect Homo sapiens
22 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
23 WDR36 134430
Co-fractionation Homo sapiens
24 BCAT1 586
Co-fractionation Homo sapiens
25 TCEA1 6917
Co-fractionation Homo sapiens
26 EFTUD2 9343
Co-fractionation Homo sapiens
27 PPP4R1L  
Affinity Capture-MS Homo sapiens
28 XPO5 57510
Co-fractionation Homo sapiens
29 SERPINE2 5270
Co-fractionation Homo sapiens
30 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 ARSG 22901
Affinity Capture-MS Homo sapiens
32 HECW2  
Affinity Capture-MS Homo sapiens
33 NAA11 84779
Affinity Capture-MS Homo sapiens
34 PTGES3 10728
Affinity Capture-MS Homo sapiens
35 RPRD1B 58490
Co-fractionation Homo sapiens
36 KLHL10  
Affinity Capture-MS Homo sapiens
37 PRKAR1B  
Co-fractionation Homo sapiens
38 RPA3 6119
Proximity Label-MS Homo sapiens
39 EEF2K  
Co-fractionation Homo sapiens
40 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
41 C1QL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 EP300 2033
Affinity Capture-MS Homo sapiens
43 ACBD3 64746
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which DUS3L is involved
No pathways found





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