Gene description for BRE
Gene name brain and reproductive organ-expressed (TNFRSF1A modulator)
Gene symbol BRE
Other names/aliases BRCC4
BRCC45
Species Homo sapiens
 Database cross references - BRE
ExoCarta ExoCarta_9577
Vesiclepedia VP_9577
Entrez Gene 9577
HGNC 1106
MIM 610497
UniProt Q9NXR7  
 BRE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for BRE
Molecular Function
    peroxisome targeting sequence binding GO:0000268 TAS
    tumor necrosis factor receptor binding GO:0005164 IDA
    protein binding GO:0005515 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
Biological Process
    regulation of DNA repair GO:0006282 NAS
    double-strand break repair GO:0006302 IBA
    double-strand break repair GO:0006302 IMP
    chromatin organization GO:0006325 IEA
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IEP
    mitotic G2 DNA damage checkpoint signaling GO:0007095 IMP
    signal transduction GO:0007165 TAS
    response to ionizing radiation GO:0010212 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    mitotic G2/M transition checkpoint GO:0044818 NAS
    positive regulation of DNA repair GO:0045739 IMP
    cell division GO:0051301 IEA
    protein K63-linked deubiquitination GO:0070536 IC
    cellular response to ionizing radiation GO:0071479 IMP
    regulation of DNA damage checkpoint GO:2000001 NAS
Subcellular Localization
    nuclear ubiquitin ligase complex GO:0000152 IDA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    BRCA1-A complex GO:0070531 IBA
    BRCA1-A complex GO:0070531 IDA
    BRCA1-A complex GO:0070531 NAS
    BRISC complex GO:0070552 IDA
 Experiment description of studies that identified BRE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BRE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PHB 5245
Affinity Capture-MS Homo sapiens
2 UGGT2 55757
Affinity Capture-MS Homo sapiens
3 SLC25A13 10165
Affinity Capture-MS Homo sapiens
4 TFG 10342
Co-fractionation Homo sapiens
5 FAM189B  
Affinity Capture-MS Homo sapiens
6 DPP9 91039
Affinity Capture-MS Homo sapiens
7 SSR3 6747
Affinity Capture-MS Homo sapiens
8 FAM175B 23172
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
9 USP7 7874
Affinity Capture-MS Homo sapiens
10 GRB2 2885
Affinity Capture-MS Homo sapiens
11 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GFI1B  
Two-hybrid Homo sapiens
13 UIMC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
15 FAS 355
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 OPTN 10133
Co-fractionation Homo sapiens
17 FAM168A  
Affinity Capture-MS Homo sapiens
18 BARD1 580
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 TTC28 23331
Affinity Capture-MS Homo sapiens
20 LRPPRC 10128
Affinity Capture-MS Homo sapiens
21 CHI3L1 1116
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ATG2A  
Affinity Capture-MS Homo sapiens
23 BRCA1 672
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
24 TNFRSF1A 7132
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 SPINK14  
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
28 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 EGFR 1956
Affinity Capture-MS Homo sapiens
30 DNAJC25 548645
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 FAM175A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 FKBP8 23770
Affinity Capture-MS Homo sapiens
34 SHMT2 6472
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
35 DHRS7B 25979
Affinity Capture-MS Homo sapiens
36 TMX3 54495
Affinity Capture-MS Homo sapiens
37 PPDPF  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
38 BABAM1 29086
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 KIAA1429 25962
Affinity Capture-MS Homo sapiens
40 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 BRCA2 675
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
42 C2orf73  
Affinity Capture-MS Homo sapiens
43 MMS19 64210
Affinity Capture-MS Homo sapiens
44 LOXL4 84171
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 DYRK1A 1859
Affinity Capture-MS Homo sapiens
47 C9orf85  
Affinity Capture-MS Homo sapiens
48 RNF166  
Affinity Capture-MS Homo sapiens
49 SUMO2 6613
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 TNS2 23371
Affinity Capture-MS Homo sapiens
51 COPS5 10987
Affinity Capture-Western Homo sapiens
52 BRCC3 79184
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
53 DCX  
Affinity Capture-MS Homo sapiens
54 MDC1  
Affinity Capture-MS Homo sapiens
55 AKAP2 11217
Co-fractionation Homo sapiens
56 Tnks2  
Affinity Capture-MS Mus musculus
57 NCOA3  
Two-hybrid Homo sapiens
58 NR3C1 2908
Proximity Label-MS Homo sapiens
59 CYTIP  
Affinity Capture-MS Homo sapiens
60 RAD51  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
61 MDM2  
Affinity Capture-Western Homo sapiens
62 RPN2 6185
Affinity Capture-MS Homo sapiens
63 TMEM192 201931
Affinity Capture-MS Homo sapiens
64 HNRNPA2B1 3181
Co-fractionation Homo sapiens
65 UBE2D1 7321
Reconstituted Complex Homo sapiens
66 PAXIP1  
Affinity Capture-MS Homo sapiens
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