Gene description for ELP3
Gene name elongator acetyltransferase complex subunit 3
Gene symbol ELP3
Other names/aliases KAT9
Species Homo sapiens
 Database cross references - ELP3
ExoCarta ExoCarta_55140
Vesiclepedia VP_55140
Entrez Gene 55140
HGNC 20696
MIM 612722
UniProt Q9H9T3  
 ELP3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ELP3
Molecular Function
    tRNA binding GO:0000049 IEA
    protein binding GO:0005515 IPI
    phosphorylase kinase regulator activity GO:0008607 IDA
    acetyltransferase activity GO:0016407 IDA
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    tRNA uridine(34) acetyltransferase activity GO:0106261 ISS
Biological Process
    neuron migration GO:0001764 ISS
    tRNA wobble uridine modification GO:0002098 IDA
    tRNA wobble uridine modification GO:0002098 NAS
    tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation GO:0002926 IBA
    tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation GO:0002926 TAS
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    regulation of translation GO:0006417 NAS
    central nervous system development GO:0007417 ISS
    positive regulation of cell migration GO:0030335 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    elongator holoenzyme complex GO:0033588 IBA
    elongator holoenzyme complex GO:0033588 IDA
    elongator holoenzyme complex GO:0033588 IPI
 Experiment description of studies that identified ELP3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ELP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
3 FTL 2512
Affinity Capture-MS Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 MPG 4350
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 IKBKAP 8518
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
7 NLN 57486
Affinity Capture-MS Homo sapiens
8 KPTN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
10 SNRNP200 23020
Co-fractionation Homo sapiens
11 FSCN1 6624
Affinity Capture-MS Homo sapiens
12 FOXP3  
Affinity Capture-MS Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 Racgap1 26934
Affinity Capture-MS Mus musculus
15 PRPS2 5634
Affinity Capture-MS Homo sapiens
16 CIAO1 9391
Affinity Capture-MS Homo sapiens
17 C9orf78 51759
Affinity Capture-MS Homo sapiens
18 WRNIP1 56897
Co-fractionation Homo sapiens
19 RIT1 6016
Negative Genetic Homo sapiens
20 HIST1H4A 8359
Biochemical Activity Homo sapiens
21 NANOG  
Affinity Capture-MS Homo sapiens
22 ISOC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 CAMK2A 815
Affinity Capture-MS Homo sapiens
24 IFI30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 HIST2H3C 126961
Biochemical Activity Homo sapiens
26 DNAL4  
Affinity Capture-MS Homo sapiens
27 QPCT 25797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PPP5C 5536
Affinity Capture-MS Homo sapiens
29 SMC6  
Affinity Capture-MS Homo sapiens
30 KTI12 112970
Affinity Capture-MS Homo sapiens
31 Soga1  
Affinity Capture-MS Mus musculus
32 CTDP1 9150
Reconstituted Complex Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 ELP6 54859
Affinity Capture-MS Homo sapiens
35 ZFYVE16  
Affinity Capture-MS Homo sapiens
36 MAGEA9  
Affinity Capture-MS Homo sapiens
37 FAM219B 57184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 POLR2A 5430
Co-purification Homo sapiens
39 ELP2 55250
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
40 Bach1 12013
Affinity Capture-MS Mus musculus
41 FAM96B 51647
Affinity Capture-MS Homo sapiens
42 WDR36 134430
Co-fractionation Homo sapiens
43 Rassf1  
Affinity Capture-MS Mus musculus
44 Kif18a  
Affinity Capture-MS Mus musculus
45 KIAA1429 25962
Affinity Capture-MS Homo sapiens
46 SKAP1 8631
Affinity Capture-MS Homo sapiens
47 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
48 MRFAP1L1  
Affinity Capture-MS Homo sapiens
49 PSME1 5720
Affinity Capture-MS Homo sapiens
50 ETV3  
Affinity Capture-MS Homo sapiens
51 MMS19 64210
Affinity Capture-MS Homo sapiens
52 RPA3 6119
Proximity Label-MS Homo sapiens
53 NTRK1 4914
Affinity Capture-MS Homo sapiens
54 PCGF6  
Affinity Capture-MS Homo sapiens
55 SSSCA1 10534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 IP6K3  
Affinity Capture-MS Homo sapiens
57 ACACB 32
Affinity Capture-MS Homo sapiens
58 DNAJA2 10294
Affinity Capture-MS Homo sapiens
59 FBXW7  
Affinity Capture-MS Homo sapiens
60 DUSP9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 PCGF1 84759
Affinity Capture-MS Homo sapiens
62 PTGES3 10728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ELP5 23587
Affinity Capture-MS Homo sapiens
64 CAMK2D 817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 C9orf72  
Affinity Capture-MS Homo sapiens
66 POU5F1  
Affinity Capture-MS Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 PCNA 5111
Affinity Capture-Western Homo sapiens
69 SLC31A1 1317
Affinity Capture-MS Homo sapiens
70 DDX58 23586
Affinity Capture-RNA Homo sapiens
71 PRPS1 5631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 PARD6A  
Affinity Capture-MS Homo sapiens
73 DCP2  
Affinity Capture-MS Homo sapiens
74 SIRT3  
Affinity Capture-MS Homo sapiens
75 LURAP1  
Affinity Capture-MS Homo sapiens
76 CAMK2B 816
Affinity Capture-MS Homo sapiens
77 TRAK1 22906
Affinity Capture-MS Homo sapiens
78 FGFR1OP  
Affinity Capture-MS Homo sapiens
79 EP300 2033
Affinity Capture-MS Homo sapiens
80 PIPSL 266971
Affinity Capture-MS Homo sapiens
81 PLEKHN1  
Affinity Capture-MS Homo sapiens
82 ELP4  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
83 HIF1A 3091
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ELP3 is involved
PathwayEvidenceSource
Chromatin modifying enzymes IEA Reactome
Chromatin organization IEA Reactome
HATs acetylate histones IEA Reactome





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