ExoCarta: Gene summary

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Gene description for DTX4
Gene name	deltex 4, E3 ubiquitin ligase
Gene symbol	DTX4
Other names/aliases	RNF155
Species	Homo sapiens
Database cross references - DTX4
ExoCarta	ExoCarta_23220
Vesiclepedia	VP_23220
Entrez Gene	23220
HGNC	29151
MIM	616110
UniProt	Q9Y2E6
DTX4 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells	34887515

Gene ontology annotations for DTX4

Molecular Function
	ubiquitin-protein transferase activity	GO:0004842	TAS
	zinc ion binding	GO:0008270	IEA
	ubiquitin protein ligase activity	GO:0061630	IBA
	ubiquitin protein ligase activity	GO:0061630	IDA
Biological Process
	Notch signaling pathway	GO:0007219	IBA
	proteasomal protein catabolic process	GO:0010498	IDA
	protein ubiquitination	GO:0016567	IBA
	regulation of type I interferon production	GO:0032479	TAS
	proteasome-mediated ubiquitin-dependent protein catabolic process	GO:0043161	IDA
	negative regulation of innate immune response	GO:0045824	IDA
	protein K48-linked ubiquitination	GO:0070936	IDA
Subcellular Localization
	nucleoplasm	GO:0005654	IBA
	cytoplasm	GO:0005737	IC
	centrosome	GO:0005813	IDA
	cytosol	GO:0005829	TAS

Experiment description of studies that identified DTX4 in sEVs

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Protein-protein interactions for DTX4

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

HPCAL1

3241

Affinity Capture-MS

Homo sapiens

FAT4

79633

Affinity Capture-MS

Homo sapiens

TBK1

29110

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

FAT3

120114

Affinity Capture-MS

Homo sapiens

NLRP4

Affinity Capture-Western

Homo sapiens

HSP90AA1

3320

Affinity Capture-Luminescence

Homo sapiens

MAP4K1

Affinity Capture-Western

Homo sapiens

FAT1

2195

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which DTX4 is involved

Pathway	Evidence	Source
Activated NOTCH1 Transmits Signal to the Nucleus	TAS	Reactome
Activated NOTCH1 Transmits Signal to the Nucleus	IEA	Reactome
Cytosolic sensors of pathogen-associated DNA	TAS	Reactome
Immune System	TAS	Reactome
Innate Immune System	TAS	Reactome
IRF3 mediated activation of type 1 IFN	TAS	Reactome
IRF3-mediated induction of type I IFN	TAS	Reactome
Regulation of innate immune responses to cytosolic DNA	TAS	Reactome
Signal Transduction	TAS	Reactome
Signal Transduction	IEA	Reactome
Signaling by NOTCH	TAS	Reactome
Signaling by NOTCH	IEA	Reactome
Signaling by NOTCH1	TAS	Reactome
Signaling by NOTCH1	IEA	Reactome
STING mediated induction of host immune responses	TAS	Reactome
ZBP1(DAI) mediated induction of type I IFNs	TAS	Reactome

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