Gene description for Lnpep
Gene name leucyl/cystinyl aminopeptidase
Gene symbol Lnpep
Other names/aliases 2010309L07Rik
4732490P18Rik
CAP
IRAP
PLAP
gp160
vp165
Species Mus musculus
 Database cross references - Lnpep
ExoCarta ExoCarta_240028
Vesiclepedia VP_240028
Entrez Gene 240028
UniProt Q8C129  
 Lnpep identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Lnpep
Molecular Function
    aminopeptidase activity GO:0004177 IMP
    aminopeptidase activity GO:0004177 ISO
    protein binding GO:0005515 IPI
    metallopeptidase activity GO:0008237 IEA
    zinc ion binding GO:0008270 IBA
    zinc ion binding GO:0008270 IEA
    peptide binding GO:0042277 IBA
    metalloaminopeptidase activity GO:0070006 IBA
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IEA
    regulation of blood pressure GO:0008217 IBA
    response to hormone GO:0009725 ISO
    neuropeptide catabolic process GO:0010813 ISO
    protein catabolic process GO:0030163 IMP
    peptide catabolic process GO:0043171 IBA
    peptide catabolic process GO:0043171 ISO
    positive regulation of blood pressure GO:0045777 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 ISO
    negative regulation of cold-induced thermogenesis GO:0120163 IMP
Subcellular Localization
    extracellular space GO:0005615 IBA
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IMP
    cytoplasmic vesicle membrane GO:0030659 IDA
    cytoplasmic vesicle membrane GO:0030659 TAS
    vesicle GO:0031982 ISO
    insulin-responsive compartment GO:0032593 ISO
    neuronal cell body GO:0043025 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Lnpep in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Lnpep
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Slc8b1  
Co-fractionation Mus musculus
2 Cd63 12512
Co-fractionation Mus musculus
3 Aspscr1  
Affinity Capture-Western Mus musculus
4 Slc2a4  
Co-fractionation Mus musculus
5 Eltd1  
Co-fractionation Mus musculus
6 Uso1 56041
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Reconstituted Complex Mus musculus
Co-fractionation Mus musculus
7 Il13ra2  
Co-fractionation Mus musculus
8 Cd47 16423
Co-fractionation Mus musculus
9 Slc9a7  
Co-fractionation Mus musculus
View the network image/svg+xml



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