Gene description for NARF
Gene name nuclear prelamin A recognition factor
Gene symbol NARF
Other names/aliases IOP2
Species Homo sapiens
 Database cross references - NARF
ExoCarta ExoCarta_26502
Vesiclepedia VP_26502
Entrez Gene 26502
HGNC 29916
MIM 605349
UniProt Q9UHQ1  
 NARF identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NARF
Molecular Function
    lamin binding GO:0005521 IBA
    lamin binding GO:0005521 IPI
Subcellular Localization
    lamin filament GO:0005638 IBA
    lamin filament GO:0005638 IDA
    nuclear lamina GO:0005652 TAS
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    nuclear lumen GO:0031981 IDA
 Experiment description of studies that identified NARF in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NARF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mtmr2  
Affinity Capture-MS Mus musculus
2 STAT5A 6776
Co-localization Homo sapiens
3 Rab11fip1  
Affinity Capture-MS Mus musculus
4 ISCA2 122961
Affinity Capture-MS Homo sapiens
5 BOLA2B 654483
Affinity Capture-MS Homo sapiens
6 SHFM1 7979
Affinity Capture-MS Homo sapiens
7 CUL3 8452
Affinity Capture-MS Homo sapiens
8 Vangl1  
Affinity Capture-MS Mus musculus
9 PARK2  
Affinity Capture-MS Homo sapiens
10 TOR1AIP1 26092
Affinity Capture-MS Homo sapiens
11 Ppp4c 56420
Affinity Capture-MS Mus musculus
12 APP 351
Reconstituted Complex Homo sapiens
13 Espl1  
Affinity Capture-MS Mus musculus
14 APEX1 328
Affinity Capture-RNA Homo sapiens
15 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ZNF691  
Affinity Capture-MS Homo sapiens
17 ACTR5 79913
Affinity Capture-MS Homo sapiens
18 CBX5 23468
Affinity Capture-MS Homo sapiens
19 Ranbp2  
Affinity Capture-MS Mus musculus
20 KCTD17 79734
Affinity Capture-MS Homo sapiens
21 PRPF4 9128
Affinity Capture-MS Homo sapiens
22 Nedd1  
Affinity Capture-MS Mus musculus
23 HS1BP3 64342
Affinity Capture-MS Homo sapiens
24 LEF1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 LMNA 4000
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
26 ENTHD2  
Affinity Capture-MS Homo sapiens
27 RGS20 8601
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NARF is involved
No pathways found





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