Gene description for BOLA2B
Gene name bolA family member 2B
Gene symbol BOLA2B
Other names/aliases BOLA2
Species Homo sapiens
 Database cross references - BOLA2B
ExoCarta ExoCarta_654483
Vesiclepedia VP_654483
Entrez Gene 654483
HGNC 32479
UniProt Q9H3K6  
 BOLA2B identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for BOLA2B
Molecular Function
    protein binding GO:0005515 IPI
    iron-sulfur cluster binding GO:0051536 IBA
    2 iron, 2 sulfur cluster binding GO:0051537 IEA
Biological Process
    intracellular iron ion homeostasis GO:0006879 IBA
    intracellular iron ion homeostasis GO:0006879 IDA
    iron-sulfur cluster assembly GO:0016226 IDA
    [2Fe-2S] cluster assembly GO:0044571 IDA
    cell redox homeostasis GO:0045454 IDA
    protein maturation by iron-sulfur cluster transfer GO:0097428 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    iron-sulfur cluster assembly complex GO:1990229 IPI
 Experiment description of studies that identified BOLA2B in sEVs
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for BOLA2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF559-ZNF177 100529215
Two-hybrid Homo sapiens
2 CGGBP1 8545
Two-hybrid Homo sapiens
3 BIRC2  
Two-hybrid Homo sapiens
4 ARRB2 409
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 XIAP  
Two-hybrid Homo sapiens
7 ZNF581  
Two-hybrid Homo sapiens
8 NARF 26502
Affinity Capture-MS Homo sapiens
9 TK1 7083
Two-hybrid Homo sapiens
10 CCDC36  
Two-hybrid Homo sapiens
11 ANKRD55  
Two-hybrid Homo sapiens
12 ZNF511  
Two-hybrid Homo sapiens
13 ADAMTSL4  
Two-hybrid Homo sapiens
14 TXNRD3  
Two-hybrid Homo sapiens
15 DLD 1738
Affinity Capture-MS Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 COX15 1355
Affinity Capture-MS Homo sapiens
18 IL16  
Two-hybrid Homo sapiens
19 KLRG2 346689
Affinity Capture-MS Homo sapiens
20 PARK7 11315
Affinity Capture-MS Homo sapiens
21 SLITRK5  
Two-hybrid Homo sapiens
22 CDK1 983
Affinity Capture-MS Homo sapiens
23 ZNF177  
Two-hybrid Homo sapiens
24 ZFP90  
Two-hybrid Homo sapiens
25 CAPZB 832
Affinity Capture-MS Homo sapiens
26 COQ2  
Affinity Capture-MS Homo sapiens
27 SOAT1 6646
Affinity Capture-MS Homo sapiens
28 HDX  
Two-hybrid Homo sapiens
29 PGK1 5230
Co-fractionation Homo sapiens
30 WDYHV1  
Two-hybrid Homo sapiens
31 DLST 1743
Affinity Capture-MS Homo sapiens
32 C10orf55  
Two-hybrid Homo sapiens
33 C1orf94  
Two-hybrid Homo sapiens
34 MEOX2  
Two-hybrid Homo sapiens
35 CCDC138  
Two-hybrid Homo sapiens
36 GLRX3 10539
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
37 ZNF620  
Two-hybrid Homo sapiens
38 C9orf72  
Affinity Capture-MS Homo sapiens
39 VDAC1 7416
Affinity Capture-MS Homo sapiens
40 BIRC7  
Two-hybrid Homo sapiens
41 CUL2 8453
Affinity Capture-MS Homo sapiens
42 RAB4B 53916
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
43 ID3  
Two-hybrid Homo sapiens
44 MBD3L1  
Two-hybrid Homo sapiens
45 ZNF774  
Two-hybrid Homo sapiens
46 ITGA4 3676
Affinity Capture-MS Homo sapiens
47 MOBP  
Two-hybrid Homo sapiens
48 ZNF688  
Two-hybrid Homo sapiens
View the network image/svg+xml



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