Experiment description of studies that identified GCNT2 in sEVs

1 Experiment ID 16 MISEV standards ✔ EM Biophysical techniques ✔ MHCII|CD86|LAMP1|LAMP2|CD63|CD81|CD82|ICAM1|TFRC Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 9685355     Organism Homo sapiens Experiment description Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes. Authors "Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ" Journal name JBC Publication year 1998 Sample B cells Sample name RN (HLA-DR15+) Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.12-1.18 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Immunoelectron Microscopy

2 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

Protein-protein interactions for GCNT2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 COL14A1 7373 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 HNRNPH1 3187 Affinity Capture-RNA Homo sapiens 3 HLA-DPB1 3115 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 CLGN 1047 Affinity Capture-MS Homo sapiens 5 PGAM2 5224 Affinity Capture-MS Homo sapiens 6 VKORC1L1 154807 Affinity Capture-MS Homo sapiens 7 FAM3C 10447 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 METTL9 51108 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 SLC35E1 79939 Affinity Capture-MS Homo sapiens 10 FNDC9   Two-hybrid Homo sapiens 11 LMF1 64788 Affinity Capture-MS Homo sapiens 12 KDELC1 79070 Affinity Capture-MS Homo sapiens 13 TMEM79   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 ATP2A1 487 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 PARK2   Affinity Capture-MS Homo sapiens 16 TRIM68 55128 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 CNPY3 10695 Affinity Capture-MS Homo sapiens 18 SYVN1 84447 Affinity Capture-MS Homo sapiens 19 FAM8A1 51439 Affinity Capture-MS Homo sapiens 20 GHITM 27069 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 COL12A1 1303 Affinity Capture-MS Homo sapiens View the network

Pathways in which GCNT2 is involved

No pathways found