Gene description for COL14A1
Gene name collagen, type XIV, alpha 1
Gene symbol COL14A1
Other names/aliases UND
Species Homo sapiens
 Database cross references - COL14A1
ExoCarta ExoCarta_7373
Vesiclepedia VP_7373
Entrez Gene 7373
HGNC 2191
MIM 120324
UniProt Q05707  
 COL14A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
 Gene ontology annotations for COL14A1
Molecular Function
    RNA binding GO:0003723 HDA
    extracellular matrix structural constituent GO:0005201 NAS
    collagen binding GO:0005518 NAS
    extracellular matrix structural constituent conferring tensile strength GO:0030020 ISS
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    protein-macromolecule adaptor activity GO:0030674 NAS
Biological Process
    ventricular cardiac muscle tissue development GO:0003229 IEA
    extracellular matrix organization GO:0030198 NAS
    collagen fibril organization GO:0030199 NAS
    homeostasis of number of cells within a tissue GO:0048873 IEA
    regulation of cell growth involved in cardiac muscle cell development GO:0061050 IEA
    cell-cell adhesion GO:0098609 NAS
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 NAS
    collagen type XIV trimer GO:0005596 NAS
    interstitial matrix GO:0005614 IBA
    extracellular space GO:0005615 HDA
    endoplasmic reticulum lumen GO:0005788 TAS
    extracellular matrix GO:0031012 NAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 ISS
 Experiment description of studies that identified COL14A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 266
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 267
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 268
MISEV standards
✘ Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors "Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
11
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for COL14A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 FGL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MMP2 4313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 METTL14  
Affinity Capture-MS Homo sapiens
6 INS 3630
Affinity Capture-MS Homo sapiens
7 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SCGB1D2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 PDGFA 5154
Affinity Capture-MS Homo sapiens
10 LAIR2  
Affinity Capture-MS Homo sapiens
11 C1QTNF9B  
Affinity Capture-MS Homo sapiens
12 PSG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 IL31  
Affinity Capture-MS Homo sapiens
14 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TP53 7157
Negative Genetic Homo sapiens
16 DCN 1634
Reconstituted Complex Homo sapiens
17 FLJ44635  
Affinity Capture-MS Homo sapiens
18 MMP9 4318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 IL12A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 COL20A1  
Affinity Capture-MS Homo sapiens
21 PDIA4 9601
Proximity Label-MS Homo sapiens
22 LCN9  
Affinity Capture-MS Homo sapiens
23 RLN1  
Affinity Capture-MS Homo sapiens
24 TMEM25  
Affinity Capture-MS Homo sapiens
25 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 P4HA2 8974
Affinity Capture-MS Homo sapiens
27 RNASE13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 COL21A1 81578
Affinity Capture-MS Homo sapiens
29 P4HA1 5033
Affinity Capture-MS Homo sapiens
30 SCGB3A1 92304
Affinity Capture-MS Homo sapiens
31 PLD4  
Affinity Capture-MS Homo sapiens
32 SIL1 64374
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CNPY3 10695
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 SFTPA2  
Affinity Capture-MS Homo sapiens
36 LRRC59 55379
Proximity Label-MS Homo sapiens
37 SCGB1C1  
Affinity Capture-MS Homo sapiens
38 FCN3 8547
Affinity Capture-MS Homo sapiens
39 NAGA 4668
Affinity Capture-MS Homo sapiens
40 OS9 10956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 RLN2  
Affinity Capture-MS Homo sapiens
42 INSL5  
Affinity Capture-MS Homo sapiens
43 APP 351
Reconstituted Complex Homo sapiens
44 GCNT2 2651
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 SYCN  
Affinity Capture-MS Homo sapiens
46 C1QTNF7  
Affinity Capture-MS Homo sapiens
47 C7orf34  
Affinity Capture-MS Homo sapiens
48 EDDM3A  
Affinity Capture-MS Homo sapiens
49 WTAP 9589
Affinity Capture-MS Homo sapiens
50 SPOCK2  
Affinity Capture-MS Homo sapiens
51 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
52 SEC61B 10952
Proximity Label-MS Homo sapiens
53 OGN 4969
Affinity Capture-MS Homo sapiens
54 FBXO2 26232
Affinity Capture-MS Homo sapiens
55 C1QTNF1 114897
Affinity Capture-MS Homo sapiens
56 SCGB2A2  
Affinity Capture-MS Homo sapiens
57 COL8A2 1296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 METTL3  
Affinity Capture-MS Homo sapiens
59 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
60 IL15  
Affinity Capture-MS Homo sapiens
61 HLA-DQB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 STC2 8614
Affinity Capture-MS Homo sapiens
63 CSF3 1440
Affinity Capture-MS Homo sapiens
64 PRELP 5549
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 PRDX2 7001
Affinity Capture-MS Homo sapiens
66 C1QC 714
Affinity Capture-MS Homo sapiens
67 FSTL1 11167
Affinity Capture-MS Homo sapiens
68 PVRL3 25945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 CD44 960
Co-purification Homo sapiens
70 GIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 C1QL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 DNAJB11 51726
Proximity Label-MS Homo sapiens
73 NMS  
Affinity Capture-MS Homo sapiens
74 TIMP4  
Affinity Capture-MS Homo sapiens
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