Gene description for MMP9
Gene name matrix metallopeptidase 9
Gene symbol MMP9
Other names/aliases CLG4B
GELB
MANDP2
MMP-9
Species Homo sapiens
 Database cross references - MMP9
ExoCarta ExoCarta_4318
Vesiclepedia VP_4318
Entrez Gene 4318
HGNC 7176
MIM 120361
UniProt P14780  
 MMP9 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Keratinocytes 19530224    
Keratinocytes 19530224    
 Gene ontology annotations for MMP9
Molecular Function
    endopeptidase activity GO:0004175 EXP
    endopeptidase activity GO:0004175 IDA
    metalloendopeptidase activity GO:0004222 IBA
    metalloendopeptidase activity GO:0004222 IDA
    metalloendopeptidase activity GO:0004222 TAS
    serine-type endopeptidase activity GO:0004252 EXP
    serine-type endopeptidase activity GO:0004252 TAS
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 TAS
    peptidase activity GO:0008233 IDA
    metallopeptidase activity GO:0008237 IDA
    zinc ion binding GO:0008270 TAS
    identical protein binding GO:0042802 IPI
Biological Process
    skeletal system development GO:0001501 IEA
    positive regulation of protein phosphorylation GO:0001934 IMP
    proteolysis GO:0006508 IDA
    apoptotic process GO:0006915 IEA
    embryo implantation GO:0007566 IEA
    cell migration GO:0016477 IGI
    cell migration GO:0016477 TAS
    extracellular matrix disassembly GO:0022617 TAS
    extracellular matrix organization GO:0030198 IBA
    macrophage differentiation GO:0030225 TAS
    collagen catabolic process GO:0030574 IBA
    collagen catabolic process GO:0030574 TAS
    endodermal cell differentiation GO:0035987 IEP
    positive regulation of apoptotic process GO:0043065 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of DNA binding GO:0043388 IDA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    ephrin receptor signaling pathway GO:0048013 TAS
    positive regulation of keratinocyte migration GO:0051549 IMP
    cellular response to lipopolysaccharide GO:0071222 TAS
    cellular response to UV-A GO:0071492 IDA
    positive regulation of release of cytochrome c from mitochondria GO:0090200 IMP
    regulation of neuroinflammatory response GO:0150077 TAS
    positive regulation of receptor binding GO:1900122 IDA
    response to amyloid-beta GO:1904645 ISS
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IMP
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IMP
    negative regulation of epithelial cell differentiation involved in kidney development GO:2000697 ISS
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
    negative regulation of cation channel activity GO:2001258 IDA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    tertiary granule lumen GO:1904724 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified MMP9 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 189
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 190
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors "Chavez-Muñoz C, Kilani RT, Ghahary A."
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MMP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ITGB1 3688
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 COL6A1 1291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CXCL5  
Biochemical Activity Homo sapiens
5 RHBDD1 84236
Affinity Capture-MS Homo sapiens
6 COLEC12 81035
Affinity Capture-MS Homo sapiens
7 TIMP1 7076
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 COL4A1 1282
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 COL6A2 1292
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 COL2A1 1280
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CXCL6  
Biochemical Activity Homo sapiens
12 GNG8  
Affinity Capture-MS Homo sapiens
13 SKP2  
Affinity Capture-MS Homo sapiens
14 COL18A1 80781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CLU 1191
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
16 COL12A1 1303
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FN1 2335
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 THBS2 7058
Affinity Capture-Western Homo sapiens
19 ITGA5 3678
Affinity Capture-Western Homo sapiens
20 USP36  
Affinity Capture-MS Homo sapiens
21 TGFBI 7045
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
22 KLK10 5655
Affinity Capture-MS Homo sapiens
23 TGFB1 7040
Co-localization Homo sapiens
Biochemical Activity Homo sapiens
24 COL5A1 1289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 THBS1 7057
Affinity Capture-Western Homo sapiens
26 EGFL8  
Affinity Capture-MS Homo sapiens
27 GBE1 2632
Affinity Capture-MS Homo sapiens
28 LCN2 3934
Affinity Capture-Western Homo sapiens
29 FBXW8 26259
Affinity Capture-MS Homo sapiens
30 SCARA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CYP3A43  
Affinity Capture-MS Homo sapiens
32 COL4A2 1284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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