Gene description for NAGA
Gene name N-acetylgalactosaminidase, alpha-
Gene symbol NAGA
Other names/aliases D22S674
GALB
Species Homo sapiens
 Database cross references - NAGA
ExoCarta ExoCarta_4668
Vesiclepedia VP_4668
Entrez Gene 4668
HGNC 7631
MIM 104170
UniProt P17050  
 NAGA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NAGA
Molecular Function
    alpha-galactosidase activity GO:0004557 IBA
    alpha-N-acetylgalactosaminidase activity GO:0008456 IDA
    protein homodimerization activity GO:0042803 IPI
Biological Process
    oligosaccharide metabolic process GO:0009311 IBA
    carbohydrate catabolic process GO:0016052 IDA
    glycoside catabolic process GO:0016139 IBA
    glycolipid catabolic process GO:0019377 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    lysosome GO:0005764 IEA
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified NAGA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NAGA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COL14A1 7373
Affinity Capture-MS Homo sapiens
2 ART5  
Affinity Capture-MS Homo sapiens
3 CANX 821
Affinity Capture-MS Homo sapiens
4 NUMA1 4926
Affinity Capture-MS Homo sapiens
5 EPDR1 54749
Affinity Capture-MS Homo sapiens
6 NAAA  
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 MAN2B1 4125
Co-fractionation Homo sapiens
9 TMEM87A 25963
Affinity Capture-MS Homo sapiens
10 FOXRED2  
Affinity Capture-MS Homo sapiens
11 AGPAT1 10554
Affinity Capture-MS Homo sapiens
12 MCCC2 64087
Co-fractionation Homo sapiens
13 NXPH4  
Affinity Capture-MS Homo sapiens
14 IDUA 3425
Affinity Capture-MS Homo sapiens
15 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TMEM223  
Affinity Capture-MS Homo sapiens
17 EPHA4 2043
Affinity Capture-MS Homo sapiens
18 UBR3  
Affinity Capture-MS Homo sapiens
19 CBLN4  
Affinity Capture-MS Homo sapiens
20 TRGV3  
Affinity Capture-MS Homo sapiens
21 METRNL 284207
Affinity Capture-MS Homo sapiens
22 PBDC1 51260
Co-fractionation Homo sapiens
23 PRG2  
Affinity Capture-MS Homo sapiens
24 PCP4  
Affinity Capture-MS Homo sapiens
25 RLN1  
Affinity Capture-MS Homo sapiens
26 MAD2L1BP  
Affinity Capture-MS Homo sapiens
27 BOC  
Affinity Capture-MS Homo sapiens
28 FBXO6 26270
Affinity Capture-MS Homo sapiens
29 EFNB3 1949
Affinity Capture-MS Homo sapiens
30 NCR3  
Affinity Capture-MS Homo sapiens
31 SLAMF1 6504
Affinity Capture-MS Homo sapiens
32 APP 351
Reconstituted Complex Homo sapiens
33 GLS 2744
Co-fractionation Homo sapiens
34 MICU2 221154
Affinity Capture-MS Homo sapiens
35 CTSG 1511
Affinity Capture-MS Homo sapiens
36 EPS8L2 64787
Co-fractionation Homo sapiens
37 LYZL1  
Affinity Capture-MS Homo sapiens
38 C1QB 713
Affinity Capture-MS Homo sapiens
39 SIRPD  
Affinity Capture-MS Homo sapiens
40 GALNT1 2589
Affinity Capture-MS Homo sapiens
41 SDF2L1 23753
Affinity Capture-MS Homo sapiens
42 CRLF1 9244
Affinity Capture-MS Homo sapiens
43 ADGRG1 9289
Affinity Capture-MS Homo sapiens
44 TIMMDC1  
Affinity Capture-MS Homo sapiens
45 ALDH3B1 221
Affinity Capture-MS Homo sapiens
46 EDN3  
Affinity Capture-MS Homo sapiens
47 NXPH3  
Affinity Capture-MS Homo sapiens
48 MAP4K5 11183
Co-fractionation Homo sapiens
49 ENPP7 339221
Affinity Capture-MS Homo sapiens
50 IL5RA  
Affinity Capture-MS Homo sapiens
51 LYPD4  
Affinity Capture-MS Homo sapiens
52 ACSL4 2182
Affinity Capture-MS Homo sapiens
53 ALKBH5 54890
Co-fractionation Homo sapiens
54 PCSK6 5046
Affinity Capture-MS Homo sapiens
55 NFXL1 152518
Affinity Capture-MS Homo sapiens
56 RMDN3 55177
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NAGA is involved
No pathways found





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