Gene description for CRLF1
Gene name cytokine receptor-like factor 1
Gene symbol CRLF1
Other names/aliases CISS
CISS1
CLF
CLF-1
NR6
zcytor5
Species Homo sapiens
 Database cross references - CRLF1
ExoCarta ExoCarta_9244
Vesiclepedia VP_9244
Entrez Gene 9244
HGNC 2364
MIM 604237
UniProt O75462  
 CRLF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CRLF1
Molecular Function
    cytokine receptor activity GO:0004896 IBA
    cytokine activity GO:0005125 IDA
    ciliary neurotrophic factor receptor binding GO:0005127 IDA
    protein binding GO:0005515 IPI
    cytokine binding GO:0019955 IBA
    cytokine binding GO:0019955 IPI
Biological Process
    ureteric bud development GO:0001657 IEA
    positive regulation of cell population proliferation GO:0008284 IBA
    positive regulation of cell population proliferation GO:0008284 IDA
    cytokine-mediated signaling pathway GO:0019221 IBA
    negative regulation of neuron apoptotic process GO:0043524 IBA
    negative regulation of neuron apoptotic process GO:0043524 IDA
    cell surface receptor signaling pathway via STAT GO:0097696 IDA
    negative regulation of motor neuron apoptotic process GO:2000672 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 TAS
    cytosol GO:0005829 TAS
    external side of plasma membrane GO:0009897 IBA
    receptor complex GO:0043235 IBA
    CRLF-CLCF1 complex GO:0097058 IBA
    CRLF-CLCF1 complex GO:0097058 IDA
 Experiment description of studies that identified CRLF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CRLF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 BMP4 652
Affinity Capture-MS Homo sapiens
3 NGLY1 55768
Affinity Capture-MS Homo sapiens
4 TOR3A 64222
Affinity Capture-MS Homo sapiens
5 LAMB1 3912
Affinity Capture-MS Homo sapiens
6 IL17RA 23765
Affinity Capture-MS Homo sapiens
7 HSPA5 3309
Affinity Capture-MS Homo sapiens
8 BTNL2  
Affinity Capture-MS Homo sapiens
9 TMEM106A  
Affinity Capture-MS Homo sapiens
10 NAAA  
Affinity Capture-MS Homo sapiens
11 CTF1  
Affinity Capture-MS Homo sapiens
12 ITIH2 3698
Affinity Capture-MS Homo sapiens
13 COL6A2 1292
Affinity Capture-MS Homo sapiens
14 NPW  
Affinity Capture-MS Homo sapiens
15 LCN6  
Affinity Capture-MS Homo sapiens
16 PCDH1 5097
Affinity Capture-MS Homo sapiens
17 HLA-F 3134
Affinity Capture-MS Homo sapiens
18 METRN 79006
Affinity Capture-MS Homo sapiens
19 EPHA7 2045
Affinity Capture-MS Homo sapiens
20 IGLL5 100423062
Affinity Capture-MS Homo sapiens
21 TMEM25  
Affinity Capture-MS Homo sapiens
22 GGH 8836
Affinity Capture-MS Homo sapiens
23 RTN4R 65078
Affinity Capture-MS Homo sapiens
24 PDGFRA 5156
Affinity Capture-MS Homo sapiens
25 PRG2  
Affinity Capture-MS Homo sapiens
26 MFAP4 4239
Affinity Capture-MS Homo sapiens
27 LGALS1 3956
Affinity Capture-MS Homo sapiens
28 RLN1  
Affinity Capture-MS Homo sapiens
29 CBLN4  
Affinity Capture-MS Homo sapiens
30 OIT3 170392
Affinity Capture-MS Homo sapiens
31 EGFL7 51162
Affinity Capture-MS Homo sapiens
32 GAA 2548
Affinity Capture-MS Homo sapiens
33 SHMT2 6472
Affinity Capture-RNA Homo sapiens
34 SLURP1  
Affinity Capture-MS Homo sapiens
35 EDEM2  
Affinity Capture-MS Homo sapiens
36 GDF15 9518
Affinity Capture-MS Homo sapiens
37 HS3ST1  
Affinity Capture-MS Homo sapiens
38 SFTPC  
Affinity Capture-MS Homo sapiens
39 KLK1 3816
Affinity Capture-MS Homo sapiens
40 MANBA 4126
Affinity Capture-MS Homo sapiens
41 SDF2L1 23753
Affinity Capture-MS Homo sapiens
42 B4GALT4 8702
Affinity Capture-MS Homo sapiens
43 HBD 3045
Affinity Capture-MS Homo sapiens
44 MOV10 4343
Affinity Capture-RNA Homo sapiens
45 PTPRS 5802
Affinity Capture-MS Homo sapiens
46 TOR1B 27348
Affinity Capture-MS Homo sapiens
47 FBXO2 26232
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 SCGB2A2  
Affinity Capture-MS Homo sapiens
49 C1orf54  
Affinity Capture-MS Homo sapiens
50 FCN3 8547
Affinity Capture-MS Homo sapiens
51 CLCF1  
Affinity Capture-Western Homo sapiens
52 BRICD5  
Affinity Capture-MS Homo sapiens
53 NPNT 255743
Affinity Capture-MS Homo sapiens
54 CLEC2B  
Affinity Capture-MS Homo sapiens
55 ENO1 2023
Affinity Capture-RNA Homo sapiens
56 GANAB 23193
Affinity Capture-MS Homo sapiens
57 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
58 NAGA 4668
Affinity Capture-MS Homo sapiens
59 SUMF1 285362
Affinity Capture-MS Homo sapiens
60 ISLR 3671
Affinity Capture-MS Homo sapiens
61 PTCH1  
Affinity Capture-MS Homo sapiens
62 CILP2  
Affinity Capture-MS Homo sapiens
63 PRSS23 11098
Affinity Capture-MS Homo sapiens
64 FGFR4 2264
Affinity Capture-MS Homo sapiens
65 TBC1D9B 23061
Affinity Capture-MS Homo sapiens
66 B3GNT3 10331
Affinity Capture-MS Homo sapiens
67 NMU  
Affinity Capture-MS Homo sapiens
68 TXNDC11 51061
Affinity Capture-MS Homo sapiens
69 ABHD14A  
Affinity Capture-MS Homo sapiens
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