Gene description for MANBA
Gene name mannosidase, beta A, lysosomal
Gene symbol MANBA
Other names/aliases MANB1
Species Homo sapiens
 Database cross references - MANBA
ExoCarta ExoCarta_4126
Vesiclepedia VP_4126
Entrez Gene 4126
HGNC 6831
MIM 609489
UniProt O00462  
 MANBA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MANBA
Molecular Function
    beta-mannosidase activity GO:0004567 IBA
    beta-mannosidase activity GO:0004567 IDA
    beta-mannosidase activity GO:0004567 TAS
    D-mannose binding GO:0005537 IEA
Biological Process
    glycoprotein catabolic process GO:0006516 IBA
    glycoprotein catabolic process GO:0006516 ISS
    oligosaccharide catabolic process GO:0009313 TAS
    protein modification process GO:0036211 NAS
Subcellular Localization
    plasma membrane GO:0005886 TAS
    azurophil granule membrane GO:0035577 TAS
    lysosomal lumen GO:0043202 TAS
 Experiment description of studies that identified MANBA in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MANBA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BRINP2  
Affinity Capture-MS Homo sapiens
2 LYZL2  
Affinity Capture-MS Homo sapiens
3 TMEM87A 25963
Affinity Capture-MS Homo sapiens
4 APOA1BP 128240
Affinity Capture-MS Homo sapiens
5 GPIHBP1  
Affinity Capture-MS Homo sapiens
6 BAGE2  
Affinity Capture-MS Homo sapiens
7 ADAMTS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CEACAM8 1088
Affinity Capture-MS Homo sapiens
9 SLURP1  
Affinity Capture-MS Homo sapiens
10 SIAE 54414
Affinity Capture-MS Homo sapiens
11 DEFA1 1667
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 LIPH 200879
Affinity Capture-MS Homo sapiens
13 PRTN3 5657
Affinity Capture-MS Homo sapiens
14 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 WNT3A 89780
Affinity Capture-MS Homo sapiens
16 PIP 5304
Affinity Capture-MS Homo sapiens
17 COL20A1  
Affinity Capture-MS Homo sapiens
18 KIRREL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 IL25  
Affinity Capture-MS Homo sapiens
20 KLK11 11012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 DNASE2B  
Affinity Capture-MS Homo sapiens
22 C1QA 712
Affinity Capture-MS Homo sapiens
23 GGH 8836
Affinity Capture-MS Homo sapiens
24 DHH  
Affinity Capture-MS Homo sapiens
25 TRGV3  
Affinity Capture-MS Homo sapiens
26 CTSG 1511
Affinity Capture-MS Homo sapiens
27 PRG2  
Affinity Capture-MS Homo sapiens
28 PI15  
Affinity Capture-MS Homo sapiens
29 RLN1  
Affinity Capture-MS Homo sapiens
30 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 C1QTNF8  
Affinity Capture-MS Homo sapiens
32 LYZL1  
Affinity Capture-MS Homo sapiens
33 FBXO6 26270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CMA1  
Affinity Capture-MS Homo sapiens
35 NCR3  
Affinity Capture-MS Homo sapiens
36 KLK2  
Affinity Capture-MS Homo sapiens
37 MFAP5  
Affinity Capture-MS Homo sapiens
38 APEX1 328
Affinity Capture-RNA Homo sapiens
39 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 DEFB136  
Affinity Capture-MS Homo sapiens
41 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 RAMP1  
Affinity Capture-MS Homo sapiens
43 C1QB 713
Affinity Capture-MS Homo sapiens
44 IDS  
Affinity Capture-MS Homo sapiens
45 PLA2G5  
Affinity Capture-MS Homo sapiens
46 SDF2L1 23753
Affinity Capture-MS Homo sapiens
47 CRLF1 9244
Affinity Capture-MS Homo sapiens
48 SPAG11A  
Affinity Capture-MS Homo sapiens
49 FBXO2 26232
Affinity Capture-MS Homo sapiens
50 PRSS37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CLEC18A  
Affinity Capture-MS Homo sapiens
52 DEFB135  
Affinity Capture-MS Homo sapiens
53 IGF2R 3482
Affinity Capture-MS Homo sapiens
54 PTPRK 5796
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 IGLV6-57  
Affinity Capture-MS Homo sapiens
56 DHFRL1  
Affinity Capture-MS Homo sapiens
57 EDN3  
Affinity Capture-MS Homo sapiens
58 PATE1  
Affinity Capture-MS Homo sapiens
59 WNT10A  
Affinity Capture-MS Homo sapiens
60 INHBE 83729
Affinity Capture-MS Homo sapiens
61 GPHA2  
Affinity Capture-MS Homo sapiens
62 ISLR 3671
Affinity Capture-MS Homo sapiens
63 CFC1  
Affinity Capture-MS Homo sapiens
64 GRIN2C 2905
Affinity Capture-MS Homo sapiens
65 PLD6  
Affinity Capture-MS Homo sapiens
66 IGFL3  
Affinity Capture-MS Homo sapiens
67 NMS  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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