Experiment description of studies that identified OIT3 in sEVs

1 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

Protein-protein interactions for OIT3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 NGLY1 55768 Affinity Capture-MS Homo sapiens 2 MOCS3 27304 Affinity Capture-MS Homo sapiens 3 WNT10B 7480 Affinity Capture-MS Homo sapiens 4 LCE1F   Two-hybrid Homo sapiens 5 ZNF101   Affinity Capture-MS Homo sapiens 6 TLL2   Affinity Capture-MS Homo sapiens 7 KRTAP11-1   Two-hybrid Homo sapiens 8 COL6A1 1291 Affinity Capture-MS Homo sapiens 9 PDIA5 10954 Affinity Capture-MS Homo sapiens 10 MYCBP2 23077 Affinity Capture-MS Homo sapiens 11 NXPH4   Affinity Capture-MS Homo sapiens 12 GTPBP6   Affinity Capture-MS Homo sapiens 13 METRN 79006 Affinity Capture-MS Homo sapiens 14 VAC14 55697 Two-hybrid Homo sapiens 15 RPS4Y2 140032 Affinity Capture-MS Homo sapiens 16 LAMB2 3913 Affinity Capture-MS Homo sapiens 17 ALDH4A1 8659 Affinity Capture-MS Homo sapiens 18 ZNF77   Affinity Capture-MS Homo sapiens 19 CELSR1 9620 Affinity Capture-MS Homo sapiens 20 RPL10 6134 Affinity Capture-MS Homo sapiens 21 ZNF146   Affinity Capture-MS Homo sapiens 22 TNFRSF6B   Affinity Capture-MS Homo sapiens 23 LTBP4 8425 Affinity Capture-MS Homo sapiens 24 ADAMTS7   Affinity Capture-MS Homo sapiens 25 CLSTN1 22883 Affinity Capture-MS Homo sapiens 26 FBXO34   Two-hybrid Homo sapiens 27 GDF15 9518 Affinity Capture-MS Homo sapiens 28 GALNT12 79695 Affinity Capture-MS Homo sapiens 29 LCE3A   Two-hybrid Homo sapiens 30 TBP   Affinity Capture-MS Homo sapiens 31 AURKB 9212 Affinity Capture-MS Homo sapiens 32 VWDE   Affinity Capture-MS Homo sapiens 33 JAG2   Affinity Capture-MS Homo sapiens 34 MYO1D 4642 Affinity Capture-MS Homo sapiens 35 CHRNA5   Affinity Capture-MS Homo sapiens 36 SLC25A4 291 Affinity Capture-MS Homo sapiens 37 PTPRK 5796 Affinity Capture-MS Homo sapiens 38 CRLF1 9244 Affinity Capture-MS Homo sapiens 39 TGFB1 7040 Affinity Capture-MS Homo sapiens 40 FBXO27   Affinity Capture-MS Homo sapiens 41 RPL23 9349 Affinity Capture-MS Homo sapiens 42 GFPT2 9945 Affinity Capture-MS Homo sapiens 43 KREMEN2   Affinity Capture-MS Homo sapiens 44 LAMA5 3911 Affinity Capture-MS Homo sapiens 45 PLAU 5328 Affinity Capture-MS Homo sapiens 46 MEOX2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 47 HOXA1 3198 Two-hybrid Homo sapiens 48 OAF 220323 Affinity Capture-MS Homo sapiens 49 SENP5   Affinity Capture-MS Homo sapiens 50 BMP1 649 Affinity Capture-MS Homo sapiens 51 TRMT2A 27037 Affinity Capture-MS Homo sapiens 52 ZNF696   Affinity Capture-MS Homo sapiens 53 CILP2   Affinity Capture-MS Homo sapiens 54 CBWD1 55871 Affinity Capture-MS Homo sapiens 55 GAS6 2621 Affinity Capture-MS Homo sapiens 56 WNT16   Affinity Capture-MS Homo sapiens 57 METRNL 284207 Affinity Capture-MS Homo sapiens 58 CHRD   Affinity Capture-MS Homo sapiens 59 CRCT1   Two-hybrid Homo sapiens 60 COL12A1 1303 Affinity Capture-MS Homo sapiens 61 LCE1C   Two-hybrid Homo sapiens 62 CCDC47 57003 Affinity Capture-MS Homo sapiens View the network

Pathways in which OIT3 is involved

No pathways found