Gene description for MOCS3
Gene name molybdenum cofactor synthesis 3
Gene symbol MOCS3
Other names/aliases UBA4
Species Homo sapiens
 Database cross references - MOCS3
ExoCarta ExoCarta_27304
Vesiclepedia VP_27304
Entrez Gene 27304
HGNC 15765
MIM 609277
UniProt O95396  
 MOCS3 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Thymus 23844026    
 Gene ontology annotations for MOCS3
Molecular Function
    thiosulfate sulfurtransferase activity GO:0004792 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    nucleotidyltransferase activity GO:0016779 IBA
    nucleotidyltransferase activity GO:0016779 IDA
    sulfurtransferase activity GO:0016783 IDA
    sulfurtransferase activity GO:0016783 IMP
    URM1 activating enzyme activity GO:0042292 IBA
    URM1 activating enzyme activity GO:0042292 IDA
    metal ion binding GO:0046872 IEA
    molybdopterin-synthase sulfurtransferase activity GO:0061604 IDA
    molybdopterin-synthase sulfurtransferase activity GO:0061604 IMP
    molybdopterin-synthase adenylyltransferase activity GO:0061605 IEA
Biological Process
    tRNA wobble uridine modification GO:0002098 IDA
    tRNA wobble position uridine thiolation GO:0002143 IBA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IDA
    Mo-molybdopterin cofactor biosynthetic process GO:0006777 IMP
    protein urmylation GO:0032447 IBA
    tRNA thio-modification GO:0034227 IDA
    tRNA thio-modification GO:0034227 IMP
    molybdopterin cofactor metabolic process GO:0043545 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MOCS3 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
3
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
4
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MOCS3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SACS 26278
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 EXOSC6 118460
Two-hybrid Homo sapiens
5 VPS16 64601
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 CA10  
Affinity Capture-MS Homo sapiens
9 HSPA6 3310
Affinity Capture-MS Homo sapiens
10 NMI  
Affinity Capture-MS Homo sapiens
11 FAS 355
Affinity Capture-MS Homo sapiens
12 NUBP2 10101
Affinity Capture-MS Homo sapiens
13 KLK5 25818
Affinity Capture-MS Homo sapiens
14 RSPH9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EFNA2  
Affinity Capture-MS Homo sapiens
16 S100A2 6273
Affinity Capture-MS Homo sapiens
17 ZNF252P-AS1  
Affinity Capture-MS Homo sapiens
18 MGARP  
Affinity Capture-MS Homo sapiens
19 C9orf78 51759
Affinity Capture-MS Homo sapiens
20 OIT3 170392
Affinity Capture-MS Homo sapiens
21 XRN2 22803
Two-hybrid Homo sapiens
22 HSPA8 3312
Affinity Capture-MS Homo sapiens
23 METTL21B  
Affinity Capture-MS Homo sapiens
24 ISX  
Affinity Capture-MS Homo sapiens
25 GXYLT1 283464
Affinity Capture-MS Homo sapiens
26 BTLA  
Affinity Capture-MS Homo sapiens
27 IL12RB1  
Affinity Capture-MS Homo sapiens
28 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KLC2 64837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 FBXL18 80028
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 FIGF 2277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 KRAS 3845
Negative Genetic Homo sapiens
33 HERC4 26091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CLPB 81570
Affinity Capture-MS Homo sapiens
35 LIN9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DYNC2H1 79659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 IRF3 3661
Affinity Capture-MS Homo sapiens
38 SULT2B1 6820
Co-fractionation Homo sapiens
39 VSX1  
Affinity Capture-MS Homo sapiens
40 ALX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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