Gene description for EFNB3
Gene name ephrin-B3
Gene symbol EFNB3
Other names/aliases EFL6
EPLG8
LERK8
Species Homo sapiens
 Database cross references - EFNB3
ExoCarta ExoCarta_1949
Vesiclepedia VP_1949
Entrez Gene 1949
HGNC 3228
MIM 602297
UniProt Q15768  
 EFNB3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for EFNB3
Molecular Function
    virus receptor activity GO:0001618 IEA
    transmembrane-ephrin receptor activity GO:0005005 TAS
    protein binding GO:0005515 IPI
    ephrin receptor binding GO:0046875 IBA
    ephrin receptor binding GO:0046875 IDA
    ephrin receptor binding GO:0046875 IPI
Biological Process
    cell-cell signaling GO:0007267 TAS
    nervous system development GO:0007399 TAS
    axon guidance GO:0007411 IBA
    adult walking behavior GO:0007628 IEA
    axon choice point recognition GO:0016198 IEA
    T cell costimulation GO:0031295 IEA
    symbiont entry into host cell GO:0046718 IEA
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IDA
    negative regulation of axonogenesis GO:0050771 ISS
    positive regulation of synaptic transmission GO:0050806 IEA
    trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission GO:0099557 IEA
    positive regulation of presynapse assembly GO:1905608 IEA
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    presynaptic membrane GO:0042734 IBA
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    postsynaptic density membrane GO:0098839 IEA
    glutamatergic synapse GO:0098978 IBA
 Experiment description of studies that identified EFNB3 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for EFNB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAGEA8  
Affinity Capture-MS Homo sapiens
2 KIAA1147  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CALML3 810
Affinity Capture-MS Homo sapiens
4 P2RX7 5027
Two-hybrid Homo sapiens
5 PICK1  
Affinity Capture-MS Homo sapiens
6 PRKCSH 5589
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 NHLRC3 387921
Affinity Capture-MS Homo sapiens
8 TCEB1 6921
Two-hybrid Homo sapiens
9 DENND6A 201627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 AATF  
Two-hybrid Homo sapiens
11 THUMPD3 25917
Affinity Capture-MS Homo sapiens
12 TUBB3 10381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HCST  
Affinity Capture-MS Homo sapiens
14 TNFSF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 EPHA4 2043
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 MTMR8  
Affinity Capture-MS Homo sapiens
17 DLG1 1739
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 PLCD3 113026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RYK 6259
Affinity Capture-MS Homo sapiens
20 YWHAZ 7534
Two-hybrid Homo sapiens
21 TUBB4B 10383
Affinity Capture-MS Homo sapiens
22 CLEC4A  
Affinity Capture-MS Homo sapiens
23 CALM1 801
Affinity Capture-MS Homo sapiens
24 PKN3 29941
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RHBDL2  
Biochemical Activity Homo sapiens
26 TUBA4A 7277
Affinity Capture-MS Homo sapiens
27 CDYL 9425
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FAM221B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HLA-DPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 TBC1D22A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LGALS1 3956
Affinity Capture-MS Homo sapiens
33 FAM115C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 IGSF8 93185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 NAGA 4668
Affinity Capture-MS Homo sapiens
36 TRIM11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MTMR1 8776
Affinity Capture-MS Homo sapiens
38 KRAS 3845
Proximity Label-MS Homo sapiens
39 MPP7 143098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 USP30  
Affinity Capture-MS Homo sapiens
41 FUCA2 2519
Affinity Capture-MS Homo sapiens
42 TUBB8 347688
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 EPHB3 2049
Reconstituted Complex Homo sapiens
44 RAD51C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 ITFG3 83986
Affinity Capture-MS Homo sapiens
46 RTEL1 51750
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 EVI5L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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