Gene description for GTF2H4
Gene name general transcription factor IIH, polypeptide 4, 52kDa
Gene symbol GTF2H4
Other names/aliases P52
TFB2
TFIIH
Species Homo sapiens
 Database cross references - GTF2H4
ExoCarta ExoCarta_2968
Vesiclepedia VP_2968
Entrez Gene 2968
HGNC 4658
MIM 601760
UniProt Q92759  
 GTF2H4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2H4
Molecular Function
    ATPase activator activity GO:0001671 IEA
    double-stranded DNA binding GO:0003690 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
Biological Process
    DNA repair GO:0006281 TAS
    nucleotide-excision repair GO:0006289 IBA
    transcription by RNA polymerase II GO:0006366 IDA
Subcellular Localization
    core TFIIH complex portion of holo TFIIH complex GO:0000438 IDA
    transcription factor TFIIH core complex GO:0000439 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIIH holo complex GO:0005675 IBA
    transcription factor TFIIH holo complex GO:0005675 IDA
    nuclear speck GO:0016607 IDA
 Experiment description of studies that identified GTF2H4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2H4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HECTD1 25831
Affinity Capture-MS Homo sapiens
2 BTAF1 9044
Co-purification Homo sapiens
3 NUCB2 4925
Co-fractionation Homo sapiens
4 EIF1AD 84285
Two-hybrid Homo sapiens
5 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
6 SIRT6  
Affinity Capture-MS Homo sapiens
7 CCNH  
Co-fractionation Homo sapiens
8 FKBP10 60681
Co-fractionation Homo sapiens
9 BRDT  
Affinity Capture-MS Homo sapiens
10 CDK7 1022
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RNF11 26994
Affinity Capture-MS Homo sapiens
12 RIT1 6016
Negative Genetic Homo sapiens
13 TUSC2  
Affinity Capture-MS Homo sapiens
14 THAP3  
Affinity Capture-MS Homo sapiens
15 REEP5 7905
Affinity Capture-MS Homo sapiens
16 BRCA1 672
Co-purification Homo sapiens
17 CD6 923
Affinity Capture-MS Homo sapiens
18 MGARP  
Co-fractionation Homo sapiens
19 PRDM11  
Affinity Capture-MS Homo sapiens
20 C9orf78 51759
Affinity Capture-MS Homo sapiens
21 MCC 4163
Affinity Capture-MS Homo sapiens
22 BRD2  
Affinity Capture-MS Homo sapiens
23 GRPEL1 80273
Co-fractionation Homo sapiens
24 GTF2H5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
25 LNPEP 4012
Affinity Capture-MS Homo sapiens
26 FAM35A  
Proximity Label-MS Homo sapiens
27 GTF2H2  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
28 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
29 GTF2B 2959
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
30 ERCC5  
Affinity Capture-MS Homo sapiens
31 TBP  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
32 PRSS8 5652
Affinity Capture-MS Homo sapiens
33 ERCC3  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
34 GTF2H2C  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
35 MED21  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
36 GTF2H3  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
37 GTF2H1 2965
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SREK1IP1  
Two-hybrid Homo sapiens
39 NT5C 30833
Affinity Capture-MS Homo sapiens
40 EAF1  
Two-hybrid Homo sapiens
41 GPR182  
Affinity Capture-MS Homo sapiens
42 TLN1 7094
Affinity Capture-MS Homo sapiens
43 GTF2E1 2960
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
44 GTF2H2C_2  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PRMT2  
Affinity Capture-MS Homo sapiens
46 RCN2 5955
Co-fractionation Homo sapiens
47 DDRGK1 65992
Affinity Capture-MS Homo sapiens
48 MNAT1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
49 Rpl35 66489
Affinity Capture-MS Mus musculus
50 MYC  
Dosage Lethality Homo sapiens
51 CDC7  
Affinity Capture-MS Homo sapiens
52 CETN2 1069
Affinity Capture-MS Homo sapiens
53 BRD3 8019
Affinity Capture-MS Homo sapiens
54 KRAS 3845
Negative Genetic Homo sapiens
55 XBP1P1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2H4 is involved
PathwayEvidenceSource
Disease IEA Reactome
DNA Repair TAS Reactome
Dual Incision in GG-NER TAS Reactome
Dual incision in TC-NER TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of Incision Complex in GG-NER TAS Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of TC-NER Pre-Incision Complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HIV Infection IEA Reactome
HIV Life Cycle IEA Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Initiation IEA Reactome
Infectious disease IEA Reactome
Late Phase of HIV Life Cycle IEA Reactome
Metabolism of RNA TAS Reactome
mRNA Capping TAS Reactome
Negative epigenetic regulation of rRNA expression IEA Reactome
NoRC negatively regulates rRNA expression IEA Reactome
Nucleotide Excision Repair TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE during HIV infection IEA Reactome
RNA Polymerase I Promoter Clearance TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Transcription TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome
RNA Polymerase I Transcription Termination TAS Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome





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