Gene description for PIK3R4
Gene name phosphoinositide-3-kinase, regulatory subunit 4
Gene symbol PIK3R4
Other names/aliases VPS15
p150
Species Homo sapiens
 Database cross references - PIK3R4
ExoCarta ExoCarta_30849
Vesiclepedia VP_30849
Entrez Gene 30849
HGNC 8982
MIM 602610
UniProt Q99570  
 PIK3R4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PIK3R4
Molecular Function
    protein kinase activity GO:0004672 NAS
    protein serine/threonine kinase activity GO:0004674 IBA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    pexophagy GO:0000425 IBA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 NAS
    protein targeting to lysosome GO:0006622 NAS
    protein targeting to vacuole GO:0006623 IBA
    regulation of autophagy GO:0010506 IDA
    regulation of macroautophagy GO:0016241 IDA
    regulation of cytokinesis GO:0032465 IMP
    receptor catabolic process GO:0032801 IMP
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IDA
    cellular response to glucose starvation GO:0042149 ISS
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IDA
    early endosome to late endosome transport GO:0045022 IDA
    late endosome to vacuole transport GO:0045324 IBA
    autophagosome maturation GO:0097352 IDA
Subcellular Localization
    late endosome GO:0005770 IBA
    late endosome GO:0005770 IDA
    autophagosome GO:0005776 IEA
    cytosol GO:0005829 TAS
    axoneme GO:0005930 ISS
    microtubule cytoskeleton GO:0015630 IDA
    membrane GO:0016020 HDA
    phagocytic vesicle membrane GO:0030670 TAS
    phosphatidylinositol 3-kinase complex, class III, type I GO:0034271 IBA
    phosphatidylinositol 3-kinase complex, class III, type II GO:0034272 IBA
    phosphatidylinositol 3-kinase complex, class III GO:0035032 IPI
    phosphatidylinositol 3-kinase complex, class III GO:0035032 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleus-vacuole junction GO:0071561 IBA
 Experiment description of studies that identified PIK3R4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PIK3R4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZRANB1 54764
Affinity Capture-MS Homo sapiens
2 HECTD1 25831
Affinity Capture-MS Homo sapiens
3 MAP1LC3C  
Affinity Capture-MS Homo sapiens
4 MAP2K2 5605
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 KIAA0226L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TUBB 203068
Affinity Capture-MS Homo sapiens
8 APLNR  
Affinity Capture-MS Homo sapiens
9 PARP1 142
Proximity Label-MS Homo sapiens
10 EEF1G 1937
Affinity Capture-MS Homo sapiens
11 DVL2 1856
Affinity Capture-Western Homo sapiens
12 RAB7A 7879
Proximity Label-MS Homo sapiens
13 LAMTOR1 55004
Proximity Label-MS Homo sapiens
14 Tuba3a 22144
Affinity Capture-MS Mus musculus
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 EGLN3  
Affinity Capture-MS Homo sapiens
17 YWHAB 7529
Affinity Capture-MS Homo sapiens
18 CCT2 10576
Affinity Capture-MS Homo sapiens
19 YWHAE 7531
Affinity Capture-MS Homo sapiens
20 RPL7 6129
Affinity Capture-MS Homo sapiens
21 MFI2 4241
Affinity Capture-MS Homo sapiens
22 NUBP2 10101
Affinity Capture-MS Homo sapiens
23 MGARP  
Affinity Capture-MS Homo sapiens
24 SLC2A1 6513
Affinity Capture-MS Homo sapiens
25 SPSB4  
Affinity Capture-MS Homo sapiens
26 LAMP2 3920
Proximity Label-MS Homo sapiens
27 CAPZB 832
Affinity Capture-MS Homo sapiens
28 ATG14  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
29 P2RY8  
Affinity Capture-MS Homo sapiens
30 RIPK4  
Affinity Capture-MS Homo sapiens
31 NXF1 10482
Affinity Capture-RNA Homo sapiens
32 Rassf1  
Affinity Capture-MS Mus musculus
33 UVRAG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB5A 5868
Proximity Label-MS Homo sapiens
35 MAPRE3  
Proximity Label-MS Homo sapiens
36 GPR17 2840
Affinity Capture-MS Homo sapiens
37 MOV10 4343
Affinity Capture-RNA Homo sapiens
38 BECN1 8678
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
39 DNAI2  
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 JADE2 23338
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 KRIT1 889
Affinity Capture-Western Homo sapiens
43 RAB9A 9367
Proximity Label-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 NRBF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
46 CKM  
Affinity Capture-MS Homo sapiens
47 KLHL34  
Affinity Capture-MS Homo sapiens
48 FLII 2314
Affinity Capture-MS Homo sapiens
49 SNW1 22938
Affinity Capture-MS Homo sapiens
50 KRAS 3845
Negative Genetic Homo sapiens
51 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
52 XRCC3  
Affinity Capture-MS Homo sapiens
53 PIK3C3 5289
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
54 FSCN1 6624
Affinity Capture-MS Homo sapiens
55 PARD6A  
Affinity Capture-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 GPR55  
Affinity Capture-MS Homo sapiens
58 FGFR1OP  
Affinity Capture-MS Homo sapiens
59 KIAA0226  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
60 RPL30 6156
Affinity Capture-MS Homo sapiens
61 C9orf72  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PIK3R4 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Antigen Presentation: Folding, assembly and peptide loading of class I MHC TAS Reactome
Autophagy TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Disease TAS Reactome
Disease IEA Reactome
Early SARS-CoV-2 Infection Events IEA Reactome
IGF1R signaling cascade TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Insulin receptor signalling cascade TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
Macroautophagy TAS Reactome
Metabolism TAS Reactome
Metabolism of lipids TAS Reactome
Phospholipid metabolism TAS Reactome
PI Metabolism TAS Reactome
PI3K Cascade TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate NADPH Oxidases TAS Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV Infections IEA Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-2 activates/modulates innate and adaptive immune responses TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 Infection IEA Reactome
SARS-CoV-2-host interactions TAS Reactome
Signal Transduction TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
Synthesis of PIPs at the early endosome membrane TAS Reactome
Synthesis of PIPs at the Golgi membrane TAS Reactome
Synthesis of PIPs at the late endosome membrane TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
Translation of Replicase and Assembly of the Replication Transcription Complex TAS Reactome
Translation of Replicase and Assembly of the Replication Transcription Complex IEA Reactome
Viral Infection Pathways TAS Reactome
Viral Infection Pathways IEA Reactome





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