Gene description for MAP2K2
Gene name mitogen-activated protein kinase kinase 2
Gene symbol MAP2K2
Other names/aliases CFC4
MAPKK2
MEK2
MKK2
PRKMK2
Species Homo sapiens
 Database cross references - MAP2K2
ExoCarta ExoCarta_5605
Vesiclepedia VP_5605
Entrez Gene 5605
HGNC 6842
MIM 601263
UniProt P36507  
 MAP2K2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
Thymus 23844026    
 Gene ontology annotations for MAP2K2
Molecular Function
    protein serine/threonine kinase activity GO:0004674 TAS
    MAP kinase kinase activity GO:0004708 IBA
    MAP kinase kinase activity GO:0004708 IDA
    protein serine/threonine/tyrosine kinase activity GO:0004712 TAS
    MAP-kinase scaffold activity GO:0005078 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    JUN kinase kinase activity GO:0008545 IEA
    PDZ domain binding GO:0030165 IDA
    histone H3Y41 kinase activity GO:0035401 IEA
    protein serine/threonine kinase activator activity GO:0043539 IDA
    metal ion binding GO:0046872 IEA
    scaffold protein binding GO:0097110 IPI
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    chromatin remodeling GO:0006338 IEA
    JNK cascade GO:0007254 IEA
    heart development GO:0007507 IEA
    positive regulation of gene expression GO:0010628 IMP
    Schwann cell development GO:0014044 IEA
    thyroid gland development GO:0030878 IEA
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    peptidyl-serine autophosphorylation GO:0036289 IDA
    ERBB2-ERBB3 signaling pathway GO:0038133 IEA
    myelination GO:0042552 IEA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    thymus development GO:0048538 IEA
    regulation of axon regeneration GO:0048679 IEA
    positive regulation of axonogenesis GO:0050772 IEA
    face development GO:0060324 IEA
    trachea formation GO:0060440 IEA
    epithelial cell proliferation involved in lung morphogenesis GO:0060502 IEA
    ERK1 and ERK2 cascade GO:0070371 IMP
    ERK1 and ERK2 cascade GO:0070371 TAS
    positive regulation of protein serine/threonine kinase activity GO:0071902 IDA
    regulation of Golgi inheritance GO:0090170 TAS
    positive regulation of cell motility GO:2000147 IEA
    regulation of early endosome to late endosome transport GO:2000641 TAS
Subcellular Localization
    extracellular region GO:0005576 NAS
    nucleus GO:0005634 TAS
    mitochondrion GO:0005739 TAS
    early endosome GO:0005769 TAS
    late endosome GO:0005770 TAS
    peroxisomal membrane GO:0005778 HDA
    endoplasmic reticulum GO:0005783 IDA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 TAS
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IDA
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 TAS
    cytoplasmic side of plasma membrane GO:0009898 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified MAP2K2 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 407
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
12
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 405
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 417
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 411
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
20
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 418
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 413
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 406
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 415
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
28
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
29
Experiment ID 408
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
30
Experiment ID 409
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
31
Experiment ID 416
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
32
Experiment ID 410
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
33
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATR  
Affinity Capture-MS Homo sapiens
2 LIG4 3981
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 SMC3 9126
Affinity Capture-MS Homo sapiens
4 WDR6 11180
Affinity Capture-MS Homo sapiens
5 SPRTN  
Affinity Capture-MS Homo sapiens
6 BAG3 9531
Affinity Capture-MS Homo sapiens
7 KSR1  
Affinity Capture-MS Homo sapiens
8 BBS7 55212
Affinity Capture-MS Homo sapiens
9 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
10 MAGED2 10916
Affinity Capture-MS Homo sapiens
11 PISD  
Affinity Capture-MS Homo sapiens
12 FAM65B  
Two-hybrid Homo sapiens
13 APBA3  
Affinity Capture-MS Homo sapiens
14 NTNG1  
Affinity Capture-MS Homo sapiens
15 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
16 ANAPC1 64682
Affinity Capture-MS Homo sapiens
17 VPS13C 54832
Affinity Capture-MS Homo sapiens
18 FPR1  
Affinity Capture-MS Homo sapiens
19 LRRN4CL 221091
Affinity Capture-MS Homo sapiens
20 DNAJB6 10049
Affinity Capture-MS Homo sapiens
21 BAG5 9529
Affinity Capture-MS Homo sapiens
22 MOS 4342
Two-hybrid Homo sapiens
23 ERF  
Affinity Capture-MS Homo sapiens
24 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 DIAPH1 1729
Co-fractionation Homo sapiens
26 HSPA2 3306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 WDR83  
Affinity Capture-Western Homo sapiens
28 FAM83D 81610
Affinity Capture-MS Homo sapiens
29 COPS5 10987
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 NFE2L2 4780
Affinity Capture-Western Homo sapiens
31 FAM83H 286077
Affinity Capture-MS Homo sapiens
32 IQGAP1 8826
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
33 SLC9A9 285195
Affinity Capture-Western Homo sapiens
34 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PIK3R4 30849
Affinity Capture-MS Homo sapiens
36 DNAJB1 3337
Affinity Capture-MS Homo sapiens
37 MACROD1 28992
Co-fractionation Homo sapiens
38 HAUS6  
Affinity Capture-MS Homo sapiens
39 TUBB4A 10382
Affinity Capture-MS Homo sapiens
40 WASL 8976
Co-fractionation Homo sapiens
41 YWHAH 7533
Affinity Capture-MS Homo sapiens
42 RHOT1 55288
Affinity Capture-MS Homo sapiens
43 EIF2A 83939
Affinity Capture-MS Homo sapiens
44 CCNDBP1 23582
Two-hybrid Homo sapiens
45 NPAS1  
Affinity Capture-MS Homo sapiens
46 FAM20C 56975
Affinity Capture-MS Homo sapiens
47 DVL2 1856
Co-fractionation Homo sapiens
48 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 TNF  
Affinity Capture-MS Homo sapiens
50 YWHAB 7529
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 PALD1 27143
Affinity Capture-MS Homo sapiens
52 MAPK1 5594
PCA Homo sapiens
Two-hybrid Homo sapiens
53 HSPBP1 23640
Affinity Capture-MS Homo sapiens
54 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 DLD 1738
Affinity Capture-MS Homo sapiens
56 FKBP5 2289
Affinity Capture-MS Homo sapiens
57 MAPK3 5595
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
58 ZNF207 7756
Two-hybrid Homo sapiens
59 MEPCE 56257
Affinity Capture-MS Homo sapiens
60 TUBA1A 7846
Affinity Capture-MS Homo sapiens
61 RPE  
Co-fractionation Homo sapiens
62 PANK4 55229
Affinity Capture-MS Homo sapiens
63 TUBA1C 84790
Affinity Capture-MS Homo sapiens
64 EXPH5  
Affinity Capture-MS Homo sapiens
65 RPA2 6118
Proximity Label-MS Homo sapiens
66 ACTL6A 86
Affinity Capture-MS Homo sapiens
67 HPS3 84343
Affinity Capture-MS Homo sapiens
68 PEX14 5195
Co-purification Homo sapiens
69 YWHAQ 10971
Affinity Capture-MS Homo sapiens
70 SMC1A 8243
Affinity Capture-MS Homo sapiens
71 Flna 192176
Two-hybrid Mus musculus
72 BRAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 EYA4 2070
Affinity Capture-Western Homo sapiens
74 MAPK8 5599
Protein-peptide Homo sapiens
75 Ksr1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
Co-fractionation Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
76 UBXN6 80700
Affinity Capture-MS Homo sapiens
77 CLN6  
Affinity Capture-MS Homo sapiens
78 PXN 5829
Co-fractionation Homo sapiens
79 HEATR6  
Affinity Capture-MS Homo sapiens
80 DNAJB14  
Affinity Capture-MS Homo sapiens
81 TUBB 203068
Affinity Capture-MS Homo sapiens
82 KIAA0100  
Affinity Capture-MS Homo sapiens
83 MUS81  
Affinity Capture-MS Homo sapiens
84 XPO1 7514
Affinity Capture-MS Homo sapiens
85 NPRL3  
Affinity Capture-MS Homo sapiens
86 BAG2 9532
Affinity Capture-MS Homo sapiens
87 DOCK7 85440
Affinity Capture-MS Homo sapiens
88 MADD 8567
Affinity Capture-MS Homo sapiens
89 NR3C1 2908
Proximity Label-MS Homo sapiens
90 EGFR 1956
Affinity Capture-MS Homo sapiens
91 OSGEP 55644
Affinity Capture-MS Homo sapiens
92 HSP90AB3P 3327
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PTK2 5747
Co-fractionation Homo sapiens
94 METTL21B  
Affinity Capture-MS Homo sapiens
95 TTF2 8458
Affinity Capture-MS Homo sapiens
96 TUBA4A 7277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 FANCA  
Affinity Capture-MS Homo sapiens
98 NRBP1 29959
Affinity Capture-MS Homo sapiens
99 HSP90AA4P 3323
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 KIF1BP 26128
Affinity Capture-MS Homo sapiens
101 RPA3 6119
Proximity Label-MS Homo sapiens
102 FAM91A1 157769
Affinity Capture-MS Homo sapiens
103 KSR2  
Affinity Capture-MS Homo sapiens
104 HSP90AA5P 730211
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 HSPA1A 3303
Affinity Capture-MS Homo sapiens
106 RICTOR 253260
Affinity Capture-MS Homo sapiens
107 RAF1 5894
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
108 CUL7 9820
Affinity Capture-MS Homo sapiens
109 P2RY10  
Affinity Capture-MS Homo sapiens
110 NCAPG2 54892
Affinity Capture-MS Homo sapiens
111 MCM3AP  
Affinity Capture-MS Homo sapiens
112 ARAF 369
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
113 ARMCX5  
Affinity Capture-MS Homo sapiens
114 PASK  
Affinity Capture-MS Homo sapiens
115 SHKBP1  
Affinity Capture-MS Homo sapiens
116 TRAF7  
Affinity Capture-MS Homo sapiens
117 MAP2K2 5605
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
118 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
119 WDR34 89891
Affinity Capture-MS Homo sapiens
120 ART3  
Affinity Capture-MS Homo sapiens
121 ZNFX1 57169
Affinity Capture-MS Homo sapiens
122 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
123 IREB2 3658
Co-fractionation Homo sapiens
124 CDC37 11140
Affinity Capture-MS Homo sapiens
125 BBS2 583
Affinity Capture-MS Homo sapiens
126 DNAJA3 9093
Affinity Capture-MS Homo sapiens
127 TUBB3 10381
Affinity Capture-MS Homo sapiens
128 DMWD  
Affinity Capture-MS Homo sapiens
129 BMPR1A 657
Affinity Capture-MS Homo sapiens
130 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
131 CNOT3 4849
Affinity Capture-MS Homo sapiens
132 DDX58 23586
Affinity Capture-RNA Homo sapiens
133 MAP2K1 5604
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
134 HNRNPK 3190
Two-hybrid Homo sapiens
135 GSTM3 2947
Affinity Capture-MS Homo sapiens
136 PI4K2B 55300
Affinity Capture-MS Homo sapiens
137 TXN 7295
Co-fractionation Homo sapiens
138 PDLIM5 10611
Co-fractionation Homo sapiens
139 PSMA5 5686
Affinity Capture-MS Homo sapiens
140 NEDD1 121441
Affinity Capture-MS Homo sapiens
141 CDK9 1025
Affinity Capture-MS Homo sapiens
142 SERPINB2 5055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 WDR59 79726
Affinity Capture-MS Homo sapiens
144 POLA1  
Affinity Capture-MS Homo sapiens
145 SERBP1 26135
Affinity Capture-MS Homo sapiens
146 SNW1 22938
Affinity Capture-MS Homo sapiens
147 HSP90AB4P 664618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 CEP170 9859
Affinity Capture-MS Homo sapiens
149 DNAI2  
Affinity Capture-MS Homo sapiens
150 HSPA1B 3304
Affinity Capture-MS Homo sapiens
151 CCRN4L  
Affinity Capture-MS Homo sapiens
152 EDRF1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAP2K2 is involved
PathwayEvidenceSource
Axon guidance TAS Reactome
Bacterial Infection Pathways TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Frs2-mediated activation TAS Reactome
Infectious disease TAS Reactome
L1CAM interactions TAS Reactome
MAP2K and MAPK activation TAS Reactome
MAPK family signaling cascades TAS Reactome
MAPK1 (ERK2) activation TAS Reactome
MAPK1/MAPK3 signaling TAS Reactome
Negative feedback regulation of MAPK pathway TAS Reactome
Negative regulation of MAPK pathway TAS Reactome
Nervous system development TAS Reactome
Oncogenic MAPK signaling TAS Reactome
Paradoxical activation of RAF signaling by kinase inactive BRAF TAS Reactome
Prolonged ERK activation events TAS Reactome
RAF activation TAS Reactome
RAF-independent MAPK1/3 activation TAS Reactome
RAF/MAP kinase cascade TAS Reactome
Signal Transduction TAS Reactome
Signal transduction by L1 TAS Reactome
Signaling by BRAF and RAF1 fusions TAS Reactome
Signaling by high-kinase activity BRAF mutants TAS Reactome
Signaling by MAP2K mutants TAS Reactome
Signaling by moderate kinase activity BRAF mutants TAS Reactome
Signaling by NTRK1 (TRKA) TAS Reactome
Signaling by NTRKs TAS Reactome
Signaling by RAF1 mutants TAS Reactome
Signaling by RAS mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling downstream of RAS mutants TAS Reactome
Signalling to ERKs TAS Reactome
Uptake and actions of bacterial toxins TAS Reactome
Uptake and function of anthrax toxins TAS Reactome





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