Gene description for ARAF
Gene name A-Raf proto-oncogene, serine/threonine kinase
Gene symbol ARAF
Other names/aliases A-RAF
ARAF1
PKS2
RAFA1
Species Homo sapiens
 Database cross references - ARAF
ExoCarta ExoCarta_369
Vesiclepedia VP_369
Entrez Gene 369
HGNC 646
MIM 311010
UniProt P10398  
 ARAF identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ARAF
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    MAP kinase kinase kinase activity GO:0004709 IBA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    metal ion binding GO:0046872 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of TOR signaling GO:0032006 IMP
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IMP
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IDA
    protein modification process GO:0036211 IEA
    negative regulation of apoptotic process GO:0043066 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARAF in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARAF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 SLC25A13 10165
Affinity Capture-MS Homo sapiens
3 GNA12 2768
Reconstituted Complex Homo sapiens
4 SPRTN  
Affinity Capture-MS Homo sapiens
5 WNK1 65125
Two-hybrid Homo sapiens
6 DYNC1I2 1781
Proximity Label-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
8 P4HA2 8974
Affinity Capture-MS Homo sapiens
9 TRIM66  
Affinity Capture-MS Homo sapiens
10 PBK  
Two-hybrid Homo sapiens
11 SLC25A1 6576
Affinity Capture-MS Homo sapiens
12 STK36  
Affinity Capture-MS Homo sapiens
13 RABGGTB 5876
Two-hybrid Homo sapiens
14 APOD 347
Affinity Capture-MS Homo sapiens
15 TRIM28 10155
Affinity Capture-MS Homo sapiens
16 DPM1 8813
Affinity Capture-MS Homo sapiens
17 CLU 1191
Affinity Capture-MS Homo sapiens
18 RAF1 5894
Affinity Capture-MS Homo sapiens
19 RAB3IP  
Affinity Capture-MS Homo sapiens
20 E2F6 1876
Two-hybrid Homo sapiens
21 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 EFEMP1 2202
Two-hybrid Homo sapiens
23 AIFM1 9131
Affinity Capture-MS Homo sapiens
24 YWHAH 7533
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ATG16L1 55054
Affinity Capture-MS Homo sapiens
26 SFN 2810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CTNNA1 1495
Affinity Capture-MS Homo sapiens
28 TIMM50 92609
Affinity Capture-MS Homo sapiens
29 KIF1BP 26128
Affinity Capture-MS Homo sapiens
30 CSNK2B 1460
Two-hybrid Homo sapiens
31 LAMTOR1 55004
Proximity Label-MS Homo sapiens
32 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 LRRC59 55379
Affinity Capture-MS Homo sapiens
34 ACTA2 59
Affinity Capture-MS Homo sapiens
35 PRPF6 24148
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
36 MYO18A 399687
Two-hybrid Homo sapiens
37 TCP1 6950
Affinity Capture-MS Homo sapiens
38 AGTRAP 57085
Two-hybrid Homo sapiens
39 PKM 5315
Co-localization Homo sapiens
40 EMD 2010
Affinity Capture-MS Homo sapiens
41 CTDP1 9150
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 TESK1 7016
Two-hybrid Homo sapiens
44 HSP90B1 7184
Affinity Capture-MS Homo sapiens
45 CPS1 1373
Two-hybrid Homo sapiens
46 IRF7  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
47 RRAS2 22800
Two-hybrid Homo sapiens
48 KDSR 2531
Affinity Capture-MS Homo sapiens
49 USMG5 84833
Affinity Capture-MS Homo sapiens
50 DDX5 1655
Affinity Capture-MS Homo sapiens
51 SLC25A22  
Affinity Capture-MS Homo sapiens
52 DNAJA1 3301
Affinity Capture-MS Homo sapiens
53 CLEC11A 6320
Affinity Capture-MS Homo sapiens
54 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 COPS3 8533
Two-hybrid Homo sapiens
56 PIK3R1 5295
Reconstituted Complex Homo sapiens
57 BRAF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
58 TECR 9524
Affinity Capture-MS Homo sapiens
59 Bub1  
Affinity Capture-MS Mus musculus
60 SDF4 51150
Affinity Capture-MS Homo sapiens
61 ITCH 83737
Affinity Capture-Western Homo sapiens
62 ATP5B 506
Affinity Capture-MS Homo sapiens
63 TIRAP  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
64 UBXN6 80700
Affinity Capture-MS Homo sapiens
65 KLHL14 57565
Affinity Capture-MS Homo sapiens
66 P3H1 64175
Affinity Capture-MS Homo sapiens
67 ZRANB1 54764
Affinity Capture-MS Homo sapiens
68 ASS1 445
Two-hybrid Homo sapiens
69 XPO1 7514
Affinity Capture-MS Homo sapiens
70 BAG2 9532
Affinity Capture-MS Homo sapiens
71 NELFCD 51497
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
72 EEF2 1938
Affinity Capture-MS Homo sapiens
73 SETD2  
Affinity Capture-MS Homo sapiens
74 CUL3 8452
Affinity Capture-MS Homo sapiens
75 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 FBXO6 26270
Affinity Capture-MS Homo sapiens
77 METTL21B  
Affinity Capture-MS Homo sapiens
78 RIPK4  
Affinity Capture-MS Homo sapiens
79 RCN1 5954
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 TUBA4A 7277
Affinity Capture-MS Homo sapiens
82 FKBP5 2289
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 RRAS 6237
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
84 CBY1  
Affinity Capture-MS Homo sapiens
85 RPA3 6119
Proximity Label-MS Homo sapiens
86 P4HB 5034
Affinity Capture-MS Homo sapiens
87 FAF2 23197
Affinity Capture-MS Homo sapiens
88 PPP6C 5537
Affinity Capture-MS Homo sapiens
89 RCN2 5955
Affinity Capture-MS Homo sapiens
90 RNF2  
Affinity Capture-MS Homo sapiens
91 HAX1  
Proximity Label-MS Homo sapiens
92 Mad2l1 56150
Affinity Capture-MS Mus musculus
93 FSCN1 6624
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
94 CCDC22 28952
Affinity Capture-MS Homo sapiens
95 STUB1 10273
Affinity Capture-MS Homo sapiens
96 HIPK4  
Affinity Capture-MS Homo sapiens
97 RPL37 6167
Affinity Capture-MS Homo sapiens
98 MAP2K2 5605
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
99 IRAK2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
100 DIDO1  
Two-hybrid Homo sapiens
101 BAD  
Biochemical Activity Homo sapiens
102 OPALIN  
Affinity Capture-MS Homo sapiens
103 DNAJA2 10294
Affinity Capture-MS Homo sapiens
104 CALU 813
Affinity Capture-MS Homo sapiens
105 CDC37 11140
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 TEKT4P2  
Two-hybrid Homo sapiens
107 CHD6 84181
Two-hybrid Homo sapiens
108 HSPB1 3315
Affinity Capture-MS Homo sapiens
109 MLH1 4292
Two-hybrid Homo sapiens
110 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
111 MAP2K1 5604
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
112 TUBB6 84617
Affinity Capture-MS Homo sapiens
113 JAK3 3718
Affinity Capture-MS Homo sapiens
114 RPL39 6170
Affinity Capture-MS Homo sapiens
115 Cdc37 12539
Affinity Capture-MS Mus musculus
116 YBX3 8531
Affinity Capture-MS Homo sapiens
117 CDK9 1025
Affinity Capture-MS Homo sapiens
118 PINK1  
Affinity Capture-MS Homo sapiens
119 RAB11A 8766
Proximity Label-MS Homo sapiens
120 SSSCA1 10534
Affinity Capture-MS Homo sapiens
121 BRD1 23774
Affinity Capture-MS Homo sapiens
122 KRAS 3845
Proximity Label-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
123 MAP1A 4130
Affinity Capture-MS Homo sapiens
124 KBTBD4  
Affinity Capture-MS Homo sapiens
125 KLHL12  
Two-hybrid Homo sapiens
126 TIMM44 10469
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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