Gene description for APAF1
Gene name apoptotic peptidase activating factor 1
Gene symbol APAF1
Other names/aliases APAF-1
CED4
Species Homo sapiens
 Database cross references - APAF1
ExoCarta ExoCarta_317
Vesiclepedia VP_317
Entrez Gene 317
HGNC 576
MIM 602233
UniProt O14727  
 APAF1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for APAF1
Molecular Function
    nucleotide binding GO:0000166 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    cysteine-type endopeptidase activator activity involved in apoptotic process GO:0008656 NAS
    heat shock protein binding GO:0031072 IEA
    identical protein binding GO:0042802 IEA
    ADP binding GO:0043531 IEA
Biological Process
    response to hypoxia GO:0001666 IEA
    kidney development GO:0001822 IEA
    neural tube closure GO:0001843 IEA
    apoptotic process GO:0006915 TAS
    nervous system development GO:0007399 TAS
    response to nutrient GO:0007584 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    cell differentiation GO:0030154 IEA
    forebrain development GO:0030900 IEA
    regulation of apoptotic process GO:0042981 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    neuron apoptotic process GO:0051402 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IEA
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    response to G1 DNA damage checkpoint signaling GO:0072432 TAS
    intrinsic apoptotic signaling pathway GO:0097193 NAS
    intrinsic apoptotic signaling pathway GO:0097193 TAS
    regulation of apoptotic DNA fragmentation GO:1902510 IEA
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
    secretory granule lumen GO:0034774 TAS
    apoptosome GO:0043293 IDA
    apoptosome GO:0043293 IPI
    apoptosome GO:0043293 TAS
    extracellular exosome GO:0070062 HDA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified APAF1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for APAF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DNAJC7 7266
Proximity Label-MS Homo sapiens
2 WDFY3 23001
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 CASP4  
Affinity Capture-Western Homo sapiens
4 PSG11  
Affinity Capture-MS Homo sapiens
5 PPT1 5538
Affinity Capture-MS Homo sapiens
6 CASP8 841
Affinity Capture-Western Homo sapiens
7 AVEN 57099
Affinity Capture-Western Homo sapiens
8 METTL21B  
Affinity Capture-MS Homo sapiens
9 NPAS1  
Affinity Capture-MS Homo sapiens
10 XIAP  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
11 PRKY  
Affinity Capture-MS Homo sapiens
12 YPEL3  
Affinity Capture-MS Homo sapiens
13 UMODL1  
Affinity Capture-MS Homo sapiens
14 CUL3 8452
Affinity Capture-MS Homo sapiens
15 CIB2  
Affinity Capture-MS Homo sapiens
16 TP53 7157
Affinity Capture-MS Homo sapiens
17 METTL10  
Affinity Capture-MS Homo sapiens
18 TRIAP1  
Affinity Capture-Western Homo sapiens
19 DNAI2  
Affinity Capture-MS Homo sapiens
20 RPEL1  
Affinity Capture-MS Homo sapiens
21 EVI5  
Affinity Capture-MS Homo sapiens
22 PLBD1 79887
Affinity Capture-MS Homo sapiens
23 TRIM35 23087
Affinity Capture-MS Homo sapiens
24 PPP1CA 5499
Affinity Capture-Western Homo sapiens
25 FAM96A  
Affinity Capture-MS Homo sapiens
26 RAD51B  
Affinity Capture-MS Homo sapiens
27 GMCL1P1  
Affinity Capture-MS Homo sapiens
28 KLK15  
Affinity Capture-MS Homo sapiens
29 NAA11 84779
Affinity Capture-MS Homo sapiens
30 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
31 FKBP9P1 360132
Affinity Capture-MS Homo sapiens
32 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
33 CUL4B 8450
Affinity Capture-Western Homo sapiens
34 ENDOV  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HSP90AA1 3320
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
36 SHMT2 6472
Affinity Capture-RNA Homo sapiens
37 LGALS7 3963
Affinity Capture-MS Homo sapiens
38 HSP90B1 7184
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 NDUFS2 4720
Co-fractionation Homo sapiens
40 AARSD1 80755
Affinity Capture-MS Homo sapiens
41 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
42 CLASRP  
Affinity Capture-MS Homo sapiens
43 PDE2A  
Affinity Capture-MS Homo sapiens
44 NPRL2  
Affinity Capture-MS Homo sapiens
45 OMD 4958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 APEX1 328
Affinity Capture-RNA Homo sapiens
47 CRYBB3  
Affinity Capture-MS Homo sapiens
48 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
49 GOLGA6L2  
Affinity Capture-MS Homo sapiens
50 MKRN2 23609
Co-fractionation Homo sapiens
51 C12orf74  
Affinity Capture-MS Homo sapiens
52 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CASP9 842
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
54 BCL2L1 598
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
55 CRYBA4  
Affinity Capture-MS Homo sapiens
56 ARL4D  
Affinity Capture-MS Homo sapiens
57 NT5C 30833
Affinity Capture-MS Homo sapiens
58 PARK2  
Affinity Capture-MS Homo sapiens
59 NLRP1  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
60 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RFXANK  
Affinity Capture-MS Homo sapiens
62 TOP1 7150
Protein-peptide Homo sapiens
63 HSPA4 3308
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
64 PPARD  
Affinity Capture-MS Homo sapiens
65 NUP107 57122
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
66 LGI1  
Affinity Capture-MS Homo sapiens
67 CYCS 54205
Affinity Capture-Western Homo sapiens
68 NFKBIB  
Affinity Capture-MS Homo sapiens
69 BANF2  
Affinity Capture-MS Homo sapiens
70 DUSP16  
Affinity Capture-MS Homo sapiens
71 CCT8L2  
Affinity Capture-MS Homo sapiens
72 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 LYPD4  
Affinity Capture-MS Homo sapiens
74 GBAP1  
Affinity Capture-MS Homo sapiens
75 HSPB8 26353
Affinity Capture-MS Homo sapiens
76 PIP5K1B 8395
Affinity Capture-MS Homo sapiens
77 CCDC8  
Affinity Capture-MS Homo sapiens
78 UBXN6 80700
Affinity Capture-MS Homo sapiens
79 APIP 51074
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here