Gene description for PIP5K1B
Gene name phosphatidylinositol-4-phosphate 5-kinase, type I, beta
Gene symbol PIP5K1B
Other names/aliases MSS4
STM7
Species Homo sapiens
 Database cross references - PIP5K1B
ExoCarta ExoCarta_8395
Vesiclepedia VP_8395
Entrez Gene 8395
HGNC 8995
MIM 602745
UniProt O14986  
 PIP5K1B identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PIP5K1B
Molecular Function
    1-phosphatidylinositol-3-phosphate 5-kinase activity GO:0000285 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 IBA
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 ISS
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 NAS
    1-phosphatidylinositol-4-phosphate 5-kinase activity GO:0016308 TAS
    phosphatidylinositol kinase activity GO:0052742 TAS
    1-phosphatidylinositol-5-kinase activity GO:0052810 TAS
Biological Process
    phosphatidylinositol biosynthetic process GO:0006661 TAS
    phosphatidylinositol phosphate biosynthetic process GO:0046854 IBA
    regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051896 TAS
Subcellular Localization
    uropod GO:0001931 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    endomembrane system GO:0012505 IEA
 Experiment description of studies that identified PIP5K1B in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PIP5K1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPRE2 10982
Affinity Capture-MS Homo sapiens
2 PIP5KL1  
Affinity Capture-Western Homo sapiens
3 APAF1 317
Affinity Capture-MS Homo sapiens
4 SPOP  
Affinity Capture-MS Homo sapiens
5 KIF20A 10112
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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