Gene description for MARK1
Gene name MAP/microtubule affinity-regulating kinase 1
Gene symbol MARK1
Other names/aliases MARK
Par-1c
Par1c
Species Homo sapiens
 Database cross references - MARK1
ExoCarta ExoCarta_4139
Vesiclepedia VP_4139
Entrez Gene 4139
HGNC 6896
MIM 606511
UniProt Q9P0L2  
 MARK1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MARK1
Molecular Function
    magnesium ion binding GO:0000287 IDA
    phosphatidylserine binding GO:0001786 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 NAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    tau protein binding GO:0048156 NAS
    tau-protein kinase activity GO:0050321 IMP
    tau-protein kinase activity GO:0050321 ISS
    tau-protein kinase activity GO:0050321 NAS
    phosphatidic acid binding GO:0070300 IDA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 ISS
    neuron migration GO:0001764 ISS
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    cytoskeleton organization GO:0007010 ISS
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IDA
    negative regulation of epithelial to mesenchymal transition GO:0010719 IDA
    regulation of neuron projection development GO:0010975 ISS
    Wnt signaling pathway GO:0016055 IEA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    regulation of dendrite development GO:0050773 ISS
    establishment of mitochondrion localization GO:0051654 ISS
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISS
    cytoskeleton GO:0005856 ISS
    plasma membrane GO:0005886 IDA
    microtubule cytoskeleton GO:0015630 TAS
    dendrite GO:0030425 IDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified MARK1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MARK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SFN 2810
Affinity Capture-MS Homo sapiens
3 PRKAA1 5562
Affinity Capture-MS Homo sapiens
4 RAB35 11021
Proximity Label-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 UTRN 7402
Affinity Capture-MS Homo sapiens
7 GJA5 2702
Two-hybrid Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 MARCKS 4082
Proximity Label-MS Homo sapiens
10 GJA1 2697
Proximity Label-MS Homo sapiens
11 CHMP4C 92421
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Affinity Capture-MS Homo sapiens
13 INCA1  
Two-hybrid Homo sapiens
14 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 USP21  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
17 CXADR 1525
Proximity Label-MS Homo sapiens
18 GPSM3  
Affinity Capture-MS Homo sapiens
19 C11orf52 91894
Proximity Label-MS Homo sapiens
20 CCDC102B  
Two-hybrid Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 Cdc20  
Affinity Capture-MS Mus musculus
23 TCL1B  
Two-hybrid Homo sapiens
24 MARK2 2011
Affinity Capture-MS Homo sapiens
25 MYC  
Affinity Capture-MS Homo sapiens
26 HECW2  
Affinity Capture-MS Homo sapiens
27 MAPT  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
FRET Homo sapiens
28 MARK1 4139
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 Map4  
Biochemical Activity Mus musculus
30 Map2  
Biochemical Activity Rattus norvegicus
31 PNMA2  
Affinity Capture-MS Homo sapiens
32 EPHA2 1969
Proximity Label-MS Homo sapiens
33 COL4A3BP 10087
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 YWHAQ 10971
Affinity Capture-MS Homo sapiens
35 KRAS 3845
Proximity Label-MS Homo sapiens
36 APBA1  
Affinity Capture-MS Homo sapiens
37 CEP85L  
Affinity Capture-MS Homo sapiens
38 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CDH19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 MECP2 4204
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MARK1 is involved
No pathways found





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