Gene description for ME2
Gene name malic enzyme 2, NAD(+)-dependent, mitochondrial
Gene symbol ME2
Other names/aliases ODS1
Species Homo sapiens
 Database cross references - ME2
ExoCarta ExoCarta_4200
Vesiclepedia VP_4200
Entrez Gene 4200
HGNC 6984
MIM 154270
UniProt P23368  
 ME2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ME2
Molecular Function
    malic enzyme activity GO:0004470 IMP
    malate dehydrogenase (decarboxylating) (NAD+) activity GO:0004471 IEA
    malate dehydrogenase (decarboxylating) (NADP+) activity GO:0004473 IBA
    oxaloacetate decarboxylase activity GO:0008948 IEA
    electron transfer activity GO:0009055 TAS
    metal ion binding GO:0046872 IEA
    NAD binding GO:0051287 IEA
Biological Process
    pyruvate metabolic process GO:0006090 IBA
    malate metabolic process GO:0006108 IBA
    regulation of NADP metabolic process GO:1902031 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified ME2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ME2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDHA1 5160
Proximity Label-MS Homo sapiens
2 PRDX4 10549
Co-fractionation Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
4 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
5 TBCC 6903
Co-fractionation Homo sapiens
6 PMPCA 23203
Proximity Label-MS Homo sapiens
7 PMPCB 9512
Proximity Label-MS Homo sapiens
8 CCDC90B  
Proximity Label-MS Homo sapiens
9 FASTKD2  
Proximity Label-MS Homo sapiens
10 TSFM 10102
Proximity Label-MS Homo sapiens
11 AUH 549
Proximity Label-MS Homo sapiens
12 IDH3A 3419
Co-fractionation Homo sapiens
13 MRRF  
Proximity Label-MS Homo sapiens
14 TBRG4 9238
Proximity Label-MS Homo sapiens
15 WDR77 79084
Affinity Capture-MS Homo sapiens
16 MTIF2 4528
Proximity Label-MS Homo sapiens
17 DDX58 23586
Affinity Capture-RNA Homo sapiens
18 LDHB 3945
Co-fractionation Homo sapiens
19 ICT1 3396
Affinity Capture-MS Homo sapiens
20 SIRT7  
Affinity Capture-MS Homo sapiens
21 RPA2 6118
Proximity Label-MS Homo sapiens
22 HSPB11 51668
Affinity Capture-MS Homo sapiens
23 TMEM184A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PAICS 10606
Co-fractionation Homo sapiens
25 COL11A1 1301
Co-fractionation Homo sapiens
26 MTG2  
Proximity Label-MS Homo sapiens
27 KDM4C  
Affinity Capture-MS Homo sapiens
28 C9orf78 51759
Affinity Capture-MS Homo sapiens
29 PYCR1 5831
Co-fractionation Homo sapiens
30 MDH2 4191
Proximity Label-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 SHMT2 6472
Co-fractionation Homo sapiens
33 YTHDF3  
Co-fractionation Homo sapiens
34 PYCR2 29920
Co-fractionation Homo sapiens
35 C21orf33  
Proximity Label-MS Homo sapiens
36 MTRF1  
Proximity Label-MS Homo sapiens
37 SSBP1 6742
Proximity Label-MS Homo sapiens
38 OAT 4942
Co-fractionation Homo sapiens
39 LRPPRC 10128
Proximity Label-MS Homo sapiens
40 CAPZB 832
Affinity Capture-MS Homo sapiens
41 HSCB 150274
Proximity Label-MS Homo sapiens
42 ACAD9 28976
Proximity Label-MS Homo sapiens
43 GFM1 85476
Proximity Label-MS Homo sapiens
44 AARS2  
Proximity Label-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 NTRK1 4914
Affinity Capture-MS Homo sapiens
47 MCM2 4171
Affinity Capture-MS Homo sapiens
48 COX8A  
Proximity Label-MS Homo sapiens
49 CCDC109B 55013
Proximity Label-MS Homo sapiens
50 MTG1  
Proximity Label-MS Homo sapiens
51 C1QBP 708
Proximity Label-MS Homo sapiens
52 HINT2 84681
Proximity Label-MS Homo sapiens
53 RCN2 5955
Co-fractionation Homo sapiens
54 TRUB2  
Proximity Label-MS Homo sapiens
55 METTL17  
Proximity Label-MS Homo sapiens
56 MRPL11 65003
Proximity Label-MS Homo sapiens
57 MTRF1L  
Proximity Label-MS Homo sapiens
58 MRPS26 64949
Proximity Label-MS Homo sapiens
59 MCUR1 63933
Proximity Label-MS Homo sapiens
60 EXD2  
Proximity Label-MS Homo sapiens
61 COX4I1 1327
Proximity Label-MS Homo sapiens
62 OGT 8473
Reconstituted Complex Homo sapiens
63 AIFM1 9131
Proximity Label-MS Homo sapiens
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