Experiment description of studies that identified PGAM4 in sEVs

1 Experiment ID 254 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name MNT-1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 259 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name A375M Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 260 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 1205Lu Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 138 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|HSP90|RAB5|LAMP2|CD9 Enriched markers ✔ HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QTOF]    PubMed ID 22723089     Organism Homo sapiens Experiment description Prostate cancer cell derived exosomes Authors "Hosseini-Beheshti E, Guns ES." Journal name MCP Publication year 2012 Sample Prostate cancer cells Sample name DU145 - Rep 2 Isolation/purification methods Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF]

5 Experiment ID 139 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|HSP90|RAB5|LAMP2|CD9 Enriched markers ✔ HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QTOF]    PubMed ID 22723089     Organism Homo sapiens Experiment description Prostate cancer cell derived exosomes Authors "Hosseini-Beheshti E, Guns ES." Journal name MCP Publication year 2012 Sample Prostate cancer cells Sample name DU145 - Rep 3 Isolation/purification methods Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF]

6 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 193 MISEV standards ✔ EM Biophysical techniques ✔ CD63|CD9 Enriched markers ✔ PHB Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 194 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of basement membrane nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

10 Experiment ID 195 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 21595033     Organism Homo sapiens Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy. Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC." Journal name Proteomics Publication year 2011 Sample Urine Sample name Urine - Patients of early IgA nephropathy Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for PGAM4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CFAP20 29105 Affinity Capture-MS Homo sapiens 2 GPX4 2879 Co-fractionation Homo sapiens 3 CCSER1   Affinity Capture-MS Homo sapiens 4 RPSAP58 388524 Affinity Capture-MS Homo sapiens 5 CCT7 10574 Affinity Capture-MS Homo sapiens 6 CCT8 10694 Affinity Capture-MS Homo sapiens 7 EPB41L4B 54566 Affinity Capture-MS Homo sapiens 8 PDCL3 79031 Affinity Capture-MS Homo sapiens 9 CCT5 22948 Affinity Capture-MS Homo sapiens 10 CCT2 10576 Affinity Capture-MS Homo sapiens 11 RIT1 6016 Negative Genetic Homo sapiens 12 PDCL 5082 Affinity Capture-MS Homo sapiens 13 PPM1B 5495 Affinity Capture-MS Homo sapiens 14 TCP1 6950 Affinity Capture-MS Homo sapiens 15 RYK 6259 Affinity Capture-MS Homo sapiens 16 CCT4 10575 Affinity Capture-MS Homo sapiens 17 CCT3 7203 Affinity Capture-MS Homo sapiens 18 CCT6B 10693 Affinity Capture-MS Homo sapiens 19 PGAM2 5224 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens Affinity Capture-MS Homo sapiens 20 TXNDC9 10190 Affinity Capture-MS Homo sapiens 21 HEY2   Affinity Capture-MS Homo sapiens 22 BPGM 669 Affinity Capture-MS Homo sapiens 23 UFL1 23376 Affinity Capture-MS Homo sapiens 24 CCT6A 908 Affinity Capture-MS Homo sapiens 25 IL2RA   Affinity Capture-MS Homo sapiens 26 BAG1 573 Affinity Capture-MS Homo sapiens 27 CCNL1   Affinity Capture-MS Homo sapiens 28 GRXCR1   Affinity Capture-MS Homo sapiens 29 SPAST 6683 Affinity Capture-MS Homo sapiens 30 DDRGK1 65992 Affinity Capture-MS Homo sapiens 31 SH2D4A 63898 Affinity Capture-MS Homo sapiens 32 PGAM1 5223 Cross-Linking-MS (XL-MS) Homo sapiens Cross-Linking-MS (XL-MS) Homo sapiens 33 DYM   Affinity Capture-MS Homo sapiens 34 TFEB   Affinity Capture-MS Homo sapiens View the network

Pathways in which PGAM4 is involved

No pathways found