Gene description for TRAT1
Gene name T cell receptor associated transmembrane adaptor 1
Gene symbol TRAT1
Other names/aliases HSPC062
TCRIM
TRIM
Species Homo sapiens
 Database cross references - TRAT1
ExoCarta ExoCarta_50852
Vesiclepedia VP_50852
Entrez Gene 50852
HGNC 30698
MIM 604962
UniProt Q6PIZ9  
 TRAT1 identified in sEVs derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for TRAT1
Molecular Function
    transmembrane receptor protein tyrosine kinase adaptor activity GO:0005068 TAS
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of receptor recycling GO:0001920 IBA
    negative regulation of receptor recycling GO:0001920 IDA
    adaptive immune response GO:0002250 IEA
    cellular defense response GO:0006968 TAS
    signal transduction GO:0007165 TAS
    cell surface receptor protein tyrosine kinase signaling pathway GO:0007169 IEA
    positive regulation of calcium-mediated signaling GO:0050850 IDA
    positive regulation of T cell receptor signaling pathway GO:0050862 IBA
    positive regulation of T cell receptor signaling pathway GO:0050862 IDA
    negative regulation of transport GO:0051051 IDA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    centriolar satellite GO:0034451 IDA
    T cell receptor complex GO:0042101 IBA
    T cell receptor complex GO:0042101 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified TRAT1 in sEVs
1
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TRAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SSR3 6747
Affinity Capture-MS Homo sapiens
2 TEX37  
Affinity Capture-MS Homo sapiens
3 GRB2 2885
Reconstituted Complex Homo sapiens
4 RHBDF2 79651
Affinity Capture-MS Homo sapiens
5 LCP2 3937
Reconstituted Complex Homo sapiens
6 YWHAE 7531
Affinity Capture-Western Homo sapiens
7 RARRES3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 PLCD3 113026
Affinity Capture-MS Homo sapiens
9 LCK 3932
Reconstituted Complex Homo sapiens
10 USP12 219333
Proximity Label-MS Homo sapiens
11 ACADVL 37
Affinity Capture-MS Homo sapiens
12 LYN 4067
Reconstituted Complex Homo sapiens
13 CD247 919
Affinity Capture-Western Homo sapiens
14 BDKRB1 623
Two-hybrid Homo sapiens
15 FYN 2534
Reconstituted Complex Homo sapiens
16 SEC22A  
Two-hybrid Homo sapiens
17 FBXW7  
Synthetic Lethality Homo sapiens
18 PLXDC2 84898
Affinity Capture-MS Homo sapiens
19 FHL2 2274
Affinity Capture-MS Homo sapiens
20 DACT3  
Affinity Capture-MS Homo sapiens
21 CAMK2B 816
Affinity Capture-MS Homo sapiens
22 GOPC 57120
Affinity Capture-MS Homo sapiens
23 IFT22 64792
Affinity Capture-MS Homo sapiens
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