Gene description for EVL
Gene name Enah/Vasp-like
Gene symbol EVL
Other names/aliases RNB6
Species Homo sapiens
 Database cross references - EVL
ExoCarta ExoCarta_51466
Vesiclepedia VP_51466
Entrez Gene 51466
HGNC 20234
UniProt Q9UI08  
 EVL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for EVL
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    profilin binding GO:0005522 IBA
    profilin binding GO:0005522 ISS
    SH3 domain binding GO:0017124 ISS
Biological Process
    actin filament organization GO:0007015 TAS
    cell surface receptor signaling pathway GO:0007166 NAS
    nervous system development GO:0007399 NAS
    axon guidance GO:0007411 IBA
    actin polymerization or depolymerization GO:0008154 IBA
    actin polymerization or depolymerization GO:0008154 ISS
    animal organ morphogenesis GO:0009887 NAS
    negative regulation of epithelial cell migration GO:0010633 IMP
    positive regulation of actin filament polymerization GO:0030838 IBA
    protein homotetramerization GO:0051289 IEA
    positive regulation of stress fiber assembly GO:0051496 IMP
    negative regulation of ruffle assembly GO:1900028 IMP
Subcellular Localization
    stress fiber GO:0001725 IEA
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    focal adhesion GO:0005925 IBA
    focal adhesion GO:0005925 ISS
    membrane GO:0016020 HDA
    lamellipodium GO:0030027 ISS
 Experiment description of studies that identified EVL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EVL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLEKHH2  
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 ENDOG  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
5 POT1  
Two-hybrid Homo sapiens
6 LYRM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PFN2 5217
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
8 ABI3 51225
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 SPTAN1 6709
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
10 CDK7 1022
Affinity Capture-MS Homo sapiens
11 TES 26136
Reconstituted Complex Homo sapiens
12 XPO1 7514
Affinity Capture-MS Homo sapiens
13 WASF2 10163
Affinity Capture-MS Homo sapiens
14 TCERG1 10915
Reconstituted Complex Homo sapiens
15 PFN1 5216
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ZYX 7791
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
17 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
18 Papss1  
Affinity Capture-MS Mus musculus
19 SH3RF1  
Two-hybrid Homo sapiens
20 MRFAP1  
Affinity Capture-MS Homo sapiens
21 XIRP1  
Two-hybrid Homo sapiens
22 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
23 ABL1 25
Reconstituted Complex Homo sapiens
24 TRIM9  
Two-hybrid Homo sapiens
25 CXorf65  
Cross-Linking-MS (XL-MS) Homo sapiens
26 TRAF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 DNMBP 23268
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
28 PRPF40A 55660
Reconstituted Complex Homo sapiens
29 EVL 51466
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
30 VASP 7408
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LYN 4067
Reconstituted Complex Homo sapiens
32 HTT 3064
Two-hybrid Homo sapiens
33 TRMT61A 115708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CEBPA  
Protein-peptide Homo sapiens
35 TERF1 7013
Two-hybrid Homo sapiens
36 CIRH1A 84916
Affinity Capture-MS Homo sapiens
37 UBP1 7342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SEMA6A  
Affinity Capture-Western Homo sapiens
39 DDX49 54555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 RPL23 9349
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 U2AF2 11338
Affinity Capture-MS Homo sapiens
42 APBB1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
43 ZNF655  
Two-hybrid Homo sapiens
44 KIAA1522 57648
Affinity Capture-MS Homo sapiens
45 FBLIM1 54751
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 LETMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 SRC 6714
Reconstituted Complex Homo sapiens
48 PVRL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 RABIF  
Co-fractionation Homo sapiens
50 ENAH 55740
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 ACTG1 71
Affinity Capture-MS Homo sapiens
52 RAD21 5885
Affinity Capture-Western Homo sapiens
53 EXD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 NAP1L5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 TACC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here