Gene description for PEX1
Gene name peroxisomal biogenesis factor 1
Gene symbol PEX1
Other names/aliases PBD1A
PBD1B
ZWS
ZWS1
Species Homo sapiens
 Database cross references - PEX1
ExoCarta ExoCarta_5189
Vesiclepedia VP_5189
Entrez Gene 5189
HGNC 8850
MIM 602136
UniProt O43933  
 PEX1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PEX1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IMP
    ATP hydrolysis activity GO:0016887 IBA
    ATP hydrolysis activity GO:0016887 IMP
    protein-containing complex binding GO:0044877 IDA
    ubiquitin-modified protein reader activity GO:0140036 IDA
    protein transporter activity GO:0140318 IDA
Biological Process
    protein targeting to peroxisome GO:0006625 IMP
    peroxisome organization GO:0007031 IMP
    protein import into peroxisome matrix GO:0016558 IBA
    protein import into peroxisome matrix GO:0016558 IMP
    protein import into peroxisome matrix, receptor recycling GO:0016562 IDA
    protein unfolding GO:0043335 IBA
    protein unfolding GO:0043335 IDA
    microtubule-based peroxisome localization GO:0060152 IMP
    microtubule-based peroxisome localization GO:0060152 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PEX1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PEX1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PEX6  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 HPSE2  
Affinity Capture-MS Homo sapiens
3 PEX26  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 FBXW11  
Affinity Capture-MS Homo sapiens
7 PUS7 54517
Affinity Capture-MS Homo sapiens
8 NPAS1  
Affinity Capture-MS Homo sapiens
9 MTCH1 23787
Proximity Label-MS Homo sapiens
10 NPRL2  
Affinity Capture-MS Homo sapiens
11 METTL10  
Affinity Capture-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 SSR2  
Affinity Capture-MS Homo sapiens
15 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 ARRDC4  
Affinity Capture-MS Homo sapiens
17 TSPYL6  
Affinity Capture-MS Homo sapiens
18 RELA 5970
Affinity Capture-MS Homo sapiens
19 PXMP2  
Proximity Label-MS Homo sapiens
20 OSTM1 28962
Affinity Capture-MS Homo sapiens
21 C12orf74  
Affinity Capture-MS Homo sapiens
22 GZMH  
Affinity Capture-MS Homo sapiens
23 TRIM66  
Affinity Capture-MS Homo sapiens
24 FAM96A  
Affinity Capture-MS Homo sapiens
25 ACTC1 70
Affinity Capture-MS Homo sapiens
26 C2orf27B  
Affinity Capture-MS Homo sapiens
27 UBA52 7311
Affinity Capture-MS Homo sapiens
28 MGARP  
Affinity Capture-MS Homo sapiens
29 MFAP4 4239
Affinity Capture-MS Homo sapiens
30 CUL3 8452
Affinity Capture-MS Homo sapiens
31 BAG2 9532
Affinity Capture-MS Homo sapiens
32 C6orf141  
Affinity Capture-MS Homo sapiens
33 PRKAB1 5564
Affinity Capture-MS Homo sapiens
34 PTPN1 5770
Proximity Label-MS Homo sapiens
35 METTL21B  
Affinity Capture-MS Homo sapiens
36 SNX21  
Affinity Capture-MS Homo sapiens
37 DUSP16  
Affinity Capture-MS Homo sapiens
38 OCIAD1 54940
Proximity Label-MS Homo sapiens
39 CHRM3 1131
Affinity Capture-MS Homo sapiens
40 PEX14 5195
Proximity Label-MS Homo sapiens
41 ISM2  
Affinity Capture-MS Homo sapiens
42 CHCHD4  
Affinity Capture-MS Homo sapiens
43 FIS1 51024
Proximity Label-MS Homo sapiens
44 EXOSC2 23404
Affinity Capture-MS Homo sapiens
45 INF2 64423
Affinity Capture-MS Homo sapiens
46 DHFR 1719
Affinity Capture-MS Homo sapiens
47 ARRDC2  
Affinity Capture-MS Homo sapiens
48 GPR45  
Affinity Capture-MS Homo sapiens
49 RAB7A 7879
Proximity Label-MS Homo sapiens
50 D2HGDH 728294
Affinity Capture-MS Homo sapiens
51 NAA11 84779
Affinity Capture-MS Homo sapiens
52 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 THBS3 7059
Affinity Capture-MS Homo sapiens
54 BRD1 23774
Affinity Capture-MS Homo sapiens
55 GNA13 10672
Affinity Capture-MS Homo sapiens
56 SMC1A 8243
Affinity Capture-MS Homo sapiens
57 CIB2  
Affinity Capture-MS Homo sapiens
58 DTWD2  
Affinity Capture-MS Homo sapiens
59 HAX1  
Proximity Label-MS Homo sapiens
60 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
61 MARCH5  
Proximity Label-MS Homo sapiens
62 DDOST 1650
Affinity Capture-MS Homo sapiens
63 CCT8L2  
Affinity Capture-MS Homo sapiens
64 PEX3 8504
Proximity Label-MS Homo sapiens
65 RHOT2 89941
Proximity Label-MS Homo sapiens
66 AKAP1 8165
Proximity Label-MS Homo sapiens
67 EXD2  
Proximity Label-MS Homo sapiens
68 UBXN6 80700
Affinity Capture-MS Homo sapiens
69 RMDN3 55177
Proximity Label-MS Homo sapiens
70 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PEX1 is involved
PathwayEvidenceSource
Peroxisomal protein import TAS Reactome
Peroxisomal protein import IEA Reactome
Protein localization TAS Reactome
Protein localization IEA Reactome





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