Gene description for PHKB
Gene name phosphorylase kinase, beta
Gene symbol PHKB
Other names/aliases -
Species Homo sapiens
 Database cross references - PHKB
ExoCarta ExoCarta_5257
Vesiclepedia VP_5257
Entrez Gene 5257
HGNC 8927
MIM 172490
UniProt Q93100  
 PHKB identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PHKB
Molecular Function
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
Biological Process
    glycogen metabolic process GO:0005977 IEA
    generation of precursor metabolites and energy GO:0006091 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    phosphorylase kinase complex GO:0005964 IBA
 Experiment description of studies that identified PHKB in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PHKB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPC 3183
Two-hybrid Homo sapiens
2 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
3 PPP1R3C  
Affinity Capture-MS Homo sapiens
4 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
5 SLU7  
Affinity Capture-MS Homo sapiens
6 MAGEA3  
Affinity Capture-MS Homo sapiens
7 TP53 7157
Affinity Capture-MS Homo sapiens
8 PRMT1 3276
Affinity Capture-MS Homo sapiens
9 DYNC1I2 1781
Proximity Label-MS Homo sapiens
10 RAMP1  
Affinity Capture-MS Homo sapiens
11 FGF12  
Affinity Capture-MS Homo sapiens
12 AURKB 9212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SAP18 10284
Affinity Capture-MS Homo sapiens
14 CARTPT  
Affinity Capture-MS Homo sapiens
15 PHKA2 5256
Affinity Capture-MS Homo sapiens
16 DOK4 55715
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 Ndc80  
Affinity Capture-MS Mus musculus
18 UBE3A 7337
Affinity Capture-MS Homo sapiens
19 C2CD4B  
Affinity Capture-MS Homo sapiens
20 EYA2  
Affinity Capture-MS Homo sapiens
21 CALM1 801
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PHKG2 5261
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 METTL21B  
Affinity Capture-MS Homo sapiens
24 CORO1C 23603
Co-fractionation Homo sapiens
25 KIAA1715 80856
Affinity Capture-MS Homo sapiens
26 VBP1 7411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RAVER1 125950
Co-fractionation Homo sapiens
28 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MPPED1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CALM2 805
Affinity Capture-MS Homo sapiens
31 C20orf27 54976
Two-hybrid Homo sapiens
32 CORO1B 57175
Co-fractionation Homo sapiens
33 Ppp1r3b  
Affinity Capture-MS Mus musculus
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 PARP1 142
Proximity Label-MS Homo sapiens
36 CALM3 808
Affinity Capture-MS Homo sapiens
37 BRAF  
Affinity Capture-MS Homo sapiens
38 PTPRT  
Proximity Label-MS Homo sapiens
39 DCP2  
Affinity Capture-MS Homo sapiens
40 PXK 54899
Affinity Capture-MS Homo sapiens
41 CAMK2B 816
Two-hybrid Homo sapiens
42 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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