Experiment description of studies that identified C20orf27 in sEVs

1 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

2 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for C20orf27

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Affinity Capture-MS Homo sapiens 2 ARHGEF7 8874 Affinity Capture-MS Homo sapiens 3 RALYL 138046 Two-hybrid Homo sapiens 4 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 5 PPP1CB 5500 Affinity Capture-MS Homo sapiens 6 PPP1R3A   Affinity Capture-MS Homo sapiens 7 TMEM69   Affinity Capture-MS Homo sapiens 8 CSK 1445 Co-fractionation Homo sapiens 9 PPP1CA 5499 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 EPB41L5 57669 Affinity Capture-MS Homo sapiens 11 CHEK2   Co-fractionation Homo sapiens 12 PPP1CC 5501 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 XRCC6 2547 Proximity Label-MS Homo sapiens 14 DYNC1LI1 51143 Proximity Label-MS Homo sapiens 15 PHKB 5257 Two-hybrid Homo sapiens 16 TERF2   Affinity Capture-MS Homo sapiens 17 BICD2 23299 Proximity Label-MS Homo sapiens 18 BICD1 636 Proximity Label-MS Homo sapiens 19 PPP1R7 5510 Affinity Capture-MS Homo sapiens 20 RPA3 6119 Proximity Label-MS Homo sapiens 21 SEC31A 22872 Co-fractionation Homo sapiens 22 FAM133A 286499 Affinity Capture-MS Homo sapiens View the network

Pathways in which C20orf27 is involved

No pathways found