Gene description for PIK3C2B
Gene name phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
Gene symbol PIK3C2B
Other names/aliases C2-PI3K
Species Homo sapiens
 Database cross references - PIK3C2B
ExoCarta ExoCarta_5287
Vesiclepedia VP_5287
Entrez Gene 5287
HGNC 8972
MIM 602838
UniProt O00750  
 PIK3C2B identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Thymus 23844026    
Urine 15326289    
 Gene ontology annotations for PIK3C2B
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IBA
    1-phosphatidylinositol-3-kinase activity GO:0016303 IDA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 IBA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 IDA
    1-phosphatidylinositol-4-phosphate 3-kinase activity GO:0035005 NAS
    phosphatidylinositol binding GO:0035091 IEA
Biological Process
    cellular response to starvation GO:0009267 IEA
    cell migration GO:0016477 IBA
    phosphatidylinositol-3-phosphate biosynthetic process GO:0036092 IBA
    phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0043491 IBA
    phosphatidylinositol-mediated signaling GO:0048015 IBA
    autophagosome organization GO:1905037 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    endoplasmic reticulum GO:0005783 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    endocytic vesicle GO:0030139 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified PIK3C2B in sEVs
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
 Protein-protein interactions for PIK3C2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 FASN 2194
Co-fractionation Homo sapiens
5 ITSN1 6453
Two-hybrid Homo sapiens
6 GRB2 2885
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 SHC1 6464
Reconstituted Complex Homo sapiens
8 LAMTOR1 55004
Proximity Label-MS Homo sapiens
9 XPO1 7514
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 SH3YL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Affinity Capture-MS Homo sapiens
13 CRK 1398
Affinity Capture-MS Homo sapiens
14 MIB1 57534
Proximity Label-MS Homo sapiens
15 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SQSTM1 8878
Proximity Label-MS Homo sapiens
17 UBC 7316
Reconstituted Complex Homo sapiens
18 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Reconstituted Complex Homo sapiens
19 RAB4A 5867
Proximity Label-MS Homo sapiens
20 ITSN2 50618
Two-hybrid Homo sapiens
21 VCP 7415
Affinity Capture-MS Homo sapiens
22 NTRK1 4914
Affinity Capture-MS Homo sapiens
23 CEP135  
Proximity Label-MS Homo sapiens
24 YWHAH 7533
Affinity Capture-MS Homo sapiens
25 TRIM27  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 EPHA2 1969
Proximity Label-MS Homo sapiens
27 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
28 YWHAG 7532
Affinity Capture-MS Homo sapiens
29 CYLD  
Affinity Capture-MS Homo sapiens
30 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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