Experiment description of studies that identified Rab11a in sEVs |
| 1 |
| Experiment ID |
73 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
HSP70|HSP90
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
18494037
|
| Organism |
Mus musculus |
| Experiment description |
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. |
| Authors |
"Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ" |
| Journal name |
ELEC
|
| Publication year |
2008 |
| Sample |
Embryonic fibroblasts |
| Sample name |
NIH3T3 |
| Isolation/purification methods |
Differential centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA] Western blotting |
|
|
| 2 |
| Experiment ID |
14 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
|
Enriched markers |
|
✔
DNM
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Western blotting
|
| PubMed ID |
16081791
|
| Organism |
Mus musculus |
| Experiment description |
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. |
| Authors |
"Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L" |
| Journal name |
JIMMU
|
| Publication year |
2005 |
| Sample |
Microglia |
| Sample name |
N9 |
| Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
| Flotation density |
1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry [LCQ DECA XP] Western blotting |
|
|
| 3 |
| Experiment ID |
188 |
| MISEV standards |
|
✔
EM
|
Biophysical techniques |
|
✔
GAPDH|UCHL1|HSP90
|
Enriched markers |
|
✘
|
Negative markers |
|
✘
|
Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
19351151
|
| Organism |
Mus musculus |
| Experiment description |
Characterization of vesicles secreted from insulinoma NIT-1 cells. |
| Authors |
"Lee HS, Jeong J, Lee KJ." |
| Journal name |
J Proteome Res
|
| Publication year |
2009 |
| Sample |
Pancreatic cells |
| Sample name |
Pancreatic beta cell (NIT-1) |
| Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|