Gene description for Apob
Gene name apolipoprotein B
Gene symbol Apob
Other names/aliases Aa1064
Ac1-060
Apo B-100
ApoB-100
ApoB-48
Species Rattus norvegicus
 Database cross references - Apob
ExoCarta ExoCarta_54225
Vesiclepedia VP_54225
Entrez Gene 54225
UniProt Q7TMA5  
 Apob identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Apob
Molecular Function
    lipid transporter activity GO:0005319 IEA
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IEA
    phospholipid binding GO:0005543 ISO
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    lipase binding GO:0035473 IEA
    lipase binding GO:0035473 ISO
    receptor ligand activity GO:0048018 IEA
    receptor ligand activity GO:0048018 ISO
    low-density lipoprotein particle receptor binding GO:0050750 IBA
    low-density lipoprotein particle receptor binding GO:0050750 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    cholesterol transfer activity GO:0120020 IBA
    cholesterol transfer activity GO:0120020 IEA
    cholesterol transfer activity GO:0120020 ISO
Biological Process
    in utero embryonic development GO:0001701 IEA
    in utero embryonic development GO:0001701 ISO
    lipid metabolic process GO:0006629 ISO
    triglyceride mobilization GO:0006642 IBA
    triglyceride mobilization GO:0006642 IEA
    triglyceride mobilization GO:0006642 ISO
    lipid transport GO:0006869 IEA
    signal transduction GO:0007165 IEA
    spermatogenesis GO:0007283 IEA
    spermatogenesis GO:0007283 ISO
    nervous system development GO:0007399 IEA
    nervous system development GO:0007399 ISO
    cholesterol metabolic process GO:0008203 IEA
    cholesterol metabolic process GO:0008203 ISO
    fertilization GO:0009566 IEA
    fertilization GO:0009566 ISO
    response to virus GO:0009615 IEA
    response to virus GO:0009615 ISO
    response to carbohydrate GO:0009743 IDA
    post-embryonic development GO:0009791 IEA
    post-embryonic development GO:0009791 ISO
    response to selenium ion GO:0010269 IEP
    positive regulation of gene expression GO:0010628 IEA
    positive regulation of gene expression GO:0010628 ISO
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISO
    positive regulation of lipid storage GO:0010884 ISO
    positive regulation of cholesterol storage GO:0010886 IEA
    positive regulation of cholesterol storage GO:0010886 ISO
    lipid catabolic process GO:0016042 ISO
    triglyceride catabolic process GO:0019433 IEA
    triglyceride catabolic process GO:0019433 IMP
    triglyceride catabolic process GO:0019433 ISO
    cholesterol transport GO:0030301 IBA
    cholesterol transport GO:0030301 ISO
    cholesterol transport GO:0030301 ISO
    flagellated sperm motility GO:0030317 IEA
    flagellated sperm motility GO:0030317 ISO
    response to estradiol GO:0032355 IEP
    response to lipopolysaccharide GO:0032496 IEP
    cholesterol efflux GO:0033344 IEA
    cholesterol efflux GO:0033344 ISO
    low-density lipoprotein particle remodeling GO:0034374 IEA
    low-density lipoprotein particle remodeling GO:0034374 ISO
    low-density lipoprotein particle clearance GO:0034383 IEA
    low-density lipoprotein particle clearance GO:0034383 ISO
    lipoprotein metabolic process GO:0042157 ISO
    lipoprotein biosynthetic process GO:0042158 IEA
    lipoprotein biosynthetic process GO:0042158 ISO
    lipoprotein catabolic process GO:0042159 IEA
    lipoprotein catabolic process GO:0042159 ISO
    cholesterol homeostasis GO:0042632 IBA
    cholesterol homeostasis GO:0042632 IEA
    cholesterol homeostasis GO:0042632 ISO
    cholesterol homeostasis GO:0042632 ISO
    lipoprotein transport GO:0042953 IBA
    lipoprotein transport GO:0042953 IEA
    lipoprotein transport GO:0042953 ISO
    regulation of cholesterol biosynthetic process GO:0045540 IEA
    regulation of cholesterol biosynthetic process GO:0045540 ISO
    artery morphogenesis GO:0048844 IEA
    artery morphogenesis GO:0048844 ISO
    establishment of localization in cell GO:0051649 IEA
    establishment of localization in cell GO:0051649 ISO
    cellular response to tumor necrosis factor GO:0071356 IEP
    cellular response to prostaglandin stimulus GO:0071379 IEP
    cellular response to lipoprotein particle stimulus GO:0071402 IEA
    cellular response to lipoprotein particle stimulus GO:0071402 ISO
Subcellular Localization
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 ISO
    lipid droplet GO:0005811 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    vesicle lumen GO:0031983 IDA
    mature chylomicron GO:0034359 IBA
    mature chylomicron GO:0034359 IEA
    mature chylomicron GO:0034359 ISO
    very-low-density lipoprotein particle GO:0034361 IBA
    very-low-density lipoprotein particle GO:0034361 IDA
    very-low-density lipoprotein particle GO:0034361 IEA
    very-low-density lipoprotein particle GO:0034361 ISO
    low-density lipoprotein particle GO:0034362 IBA
    low-density lipoprotein particle GO:0034362 IDA
    low-density lipoprotein particle GO:0034362 IEA
    low-density lipoprotein particle GO:0034362 ISO
    intermediate-density lipoprotein particle GO:0034363 IEA
    intermediate-density lipoprotein particle GO:0034363 ISO
    high-density lipoprotein particle GO:0034364 IDA
    chylomicron GO:0042627 IEA
    chylomicron GO:0042627 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    endoplasmic reticulum exit site GO:0070971 IEA
    endoplasmic reticulum exit site GO:0070971 ISO
 Experiment description of studies that identified Apob in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Apob
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CELF2 10659
Affinity Capture-RNA Homo sapiens
View the network image/svg+xml
 Pathways in which Apob is involved
PathwayEvidenceSource
Binding and Uptake of Ligands by Scavenger Receptors IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Chylomicron assembly IEA Reactome
Chylomicron clearance IEA Reactome
Chylomicron remodeling IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Heme signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism IEA Reactome
Metabolism of fat-soluble vitamins IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of vitamins and cofactors IEA Reactome
Plasma lipoprotein assembly IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Plasma lipoprotein remodeling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
Post-translational protein modification IEA Reactome
Post-translational protein phosphorylation IEA Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) IEA Reactome
Regulation of TLR by endogenous ligand IEA Reactome
Retinoid metabolism and transport IEA Reactome
Scavenging by Class A Receptors IEA Reactome
Scavenging by Class B Receptors IEA Reactome
Scavenging by Class H Receptors IEA Reactome
Sensory Perception IEA Reactome
Toll-like Receptor Cascades IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
Visual phototransduction IEA Reactome
VLDL assembly IEA Reactome
VLDL clearance IEA Reactome





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