ExoCarta: Gene summary

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Gene description for ESRP1
Gene name	epithelial splicing regulatory protein 1
Gene symbol	ESRP1
Other names/aliases	RBM35A RMB35A
Species	Homo sapiens
Database cross references - ESRP1
ExoCarta	ExoCarta_54845
Vesiclepedia	VP_54845
Entrez Gene	54845
HGNC	25966
MIM	612959
UniProt	Q6NXG1
ESRP1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells	34887515
Ovarian cancer cells	23333927
Ovarian cancer cells	23333927
Squamous carcinoma cells	20124223

Gene ontology annotations for ESRP1

Molecular Function
	mRNA binding	GO:0003729	IBA
	mRNA binding	GO:0003729	IDA
	protein binding	GO:0005515	IPI
Biological Process
	mRNA processing	GO:0006397	IEA
	RNA splicing	GO:0008380	IEA
	regulation of inner ear auditory receptor cell fate specification	GO:0042669	ISS
	regulation of RNA splicing	GO:0043484	IBA
	regulation of RNA splicing	GO:0043484	IDA
Subcellular Localization
	nucleus	GO:0005634	IDA
	nucleoplasm	GO:0005654	IBA
	nucleoplasm	GO:0005654	IDA
	nucleoplasm	GO:0005654	TAS
	nuclear body	GO:0016604	IDA
	ribonucleoprotein complex	GO:1990904	IBA

Experiment description of studies that identified ESRP1 in sEVs

Experiment ID

1203

MISEV standards
✔ EM	Biophysical techniques
✔ SDCBP\|FLOT1\|CD9\|CD81\|CD63\|EPCAM\|GAPDH\|LAMP1\|TFRC\|CD151\|CD82\|LAMP2\|RAB35\|TSG101\|FLOT2\|RAB5B\|ICAM1\|RAB5A	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

34887515

Organism

Homo sapiens

Experiment description

Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets

Authors

"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."

Journal name

Nat Cell Biol

Publication year

2021

Sample

Colorectal cancer cells

Sample name

DiFi

Isolation/purification methods

Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation

Flotation density

Molecules identified in the study

Protein
miRNA

Methods used in the study

Western blotting
Mass spectrometry
RNA sequencing

Experiment ID

211

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ cytochrome c\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

IGROV1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

212

MISEV standards
✔ CEM	Biophysical techniques
✔ TSG101\|Alix\|EpCAM\|TFRC	Enriched markers
✔ Cytochrome C\|GOLGA2	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

23333927

Organism

Homo sapiens

Experiment description

Characterization and proteomic analysis of ovarian cancer-derived exosomes.

Authors

"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."

Journal name

J Proteomics

Publication year

2013

Sample

Ovarian cancer cells

Sample name

OVCAR-3

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.09-1.15 g/mL

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Experiment ID

191

MISEV standards
✘	Biophysical techniques
✔ Alix\|CD81\|CD9	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

20124223

Organism

Homo sapiens

Experiment description

Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.

Authors

"Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."

Journal name

Mol Cell Proteomics

Publication year

2010

Sample

Squamous carcinoma cells

Sample name

Squamous carcinoma cell (A431)

Isolation/purification methods

Differential centrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Mass spectrometry

Protein-protein interactions for ESRP1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

KRTAP19-5

Two-hybrid

Homo sapiens

ELAVL2

1993

Affinity Capture-MS

Homo sapiens

ATXN1

6310

Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens

ALG13

79868

Two-hybrid

Homo sapiens

RBPMS

11030

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PRR20C

Two-hybrid

Homo sapiens

Rbm38

Two-hybrid

Mus musculus

PRR20A

Two-hybrid

Homo sapiens

KIF14

9928

Affinity Capture-MS

Homo sapiens

THUMPD3

25917

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

CIT

11113

Affinity Capture-MS

Homo sapiens

KIF20A

10112

Affinity Capture-MS

Homo sapiens

ATXN2

6311

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ARID5A

Two-hybrid

Homo sapiens

ZNF703

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MEN1

4221

Affinity Capture-MS

Homo sapiens

Rbm47

Two-hybrid

Mus musculus

PRR20D

Two-hybrid

Homo sapiens

RGPD3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

KLHDC7B

Two-hybrid

Homo sapiens

OXER1

Two-hybrid

Homo sapiens

ZC3H10

Two-hybrid

Homo sapiens

ESR1

Affinity Capture-MS

Homo sapiens

DAZAP2

Two-hybrid

Homo sapiens

BIRC6

57448

Affinity Capture-RNA

Homo sapiens

PRR20B

Two-hybrid

Homo sapiens

PRR20E

Two-hybrid

Homo sapiens

RGPD5

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HELZ

9931

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RBPMS2

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

RNF111

54778

Affinity Capture-Western

Homo sapiens

PRC1

9055

Affinity Capture-MS

Homo sapiens

GPATCH8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MRPS24

64951

Two-hybrid

Homo sapiens

H19

283120

Protein-RNA

Homo sapiens

RNF2

Affinity Capture-MS

Homo sapiens

BMI1

Affinity Capture-MS

Homo sapiens

MED25

Two-hybrid

Homo sapiens

RGPD8

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RANBP2

5903

Affinity Capture-MS

Homo sapiens

PAK6

56924

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which ESRP1 is involved

Pathway	Evidence	Source
Disease	TAS	Reactome
Diseases of signal transduction by growth factor receptors and second messengers	TAS	Reactome
FGFR2 alternative splicing	TAS	Reactome
Oncogenic MAPK signaling	TAS	Reactome
Signal Transduction	TAS	Reactome
Signaling by BRAF and RAF1 fusions	TAS	Reactome
Signaling by FGFR	TAS	Reactome
Signaling by FGFR2	TAS	Reactome
Signaling by Receptor Tyrosine Kinases	TAS	Reactome

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