Gene description for ZWILCH
Gene name zwilch kinetochore protein
Gene symbol ZWILCH
Other names/aliases KNTC1AP
hZwilch
Species Homo sapiens
 Database cross references - ZWILCH
ExoCarta ExoCarta_55055
Vesiclepedia VP_55055
Entrez Gene 55055
HGNC 25468
MIM 609984
UniProt Q9H900  
 ZWILCH identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ZWILCH
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    mitotic spindle assembly checkpoint signaling GO:0007094 IBA
    mitotic spindle assembly checkpoint signaling GO:0007094 IMP
    protein localization to kinetochore GO:0034501 IBA
    protein localization to kinetochore GO:0034501 IMP
    cell division GO:0051301 IEA
    regulation of attachment of spindle microtubules to kinetochore GO:0051988 NAS
Subcellular Localization
    kinetochore GO:0000776 IDA
    kinetochore microtubule GO:0005828 NAS
    cytosol GO:0005829 TAS
    RZZ complex GO:1990423 IBA
    RZZ complex GO:1990423 IDA
    RZZ complex GO:1990423 IPI
 Experiment description of studies that identified ZWILCH in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ZWILCH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Co-fractionation Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 EFNA4  
Affinity Capture-MS Homo sapiens
5 UBE2A  
Co-fractionation Homo sapiens
6 GPR17 2840
Affinity Capture-MS Homo sapiens
7 BTNL9  
Affinity Capture-MS Homo sapiens
8 OSTM1 28962
Affinity Capture-MS Homo sapiens
9 NPTN 27020
Affinity Capture-MS Homo sapiens
10 Zw10  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
11 TMEM206 55248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CLN5  
Affinity Capture-MS Homo sapiens
13 IQCF1  
Affinity Capture-MS Homo sapiens
14 NAT14 57106
Affinity Capture-MS Homo sapiens
15 APEX1 328
Affinity Capture-RNA Homo sapiens
16 ZW10 9183
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
17 C12orf49  
Affinity Capture-MS Homo sapiens
18 SNX8 29886
Co-fractionation Homo sapiens
19 SEPT8 23176
Co-fractionation Homo sapiens
20 GPR182  
Affinity Capture-MS Homo sapiens
21 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SEPT7 989
Co-fractionation Homo sapiens
23 SLC2A9  
Affinity Capture-MS Homo sapiens
24 ITFG3 83986
Affinity Capture-MS Homo sapiens
25 HIST2H2BE 8349
Affinity Capture-MS Homo sapiens
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