Gene description for MTPAP
Gene name mitochondrial poly(A) polymerase
Gene symbol MTPAP
Other names/aliases PAPD1
SPAX4
Species Homo sapiens
 Database cross references - MTPAP
ExoCarta ExoCarta_55149
Vesiclepedia VP_55149
Entrez Gene 55149
HGNC 25532
MIM 613669
UniProt Q9NVV4  
 MTPAP identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MTPAP
Molecular Function
    magnesium ion binding GO:0000287 IDA
    UTP binding GO:0002134 IDA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    manganese ion binding GO:0030145 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
    poly(A) RNA polymerase activity GO:1990817 IBA
    poly(A) RNA polymerase activity GO:1990817 IDA
Biological Process
    mitochondrial RNA 3'-end processing GO:0000965 IDA
    mRNA processing GO:0006397 IEA
    RNA 3'-end processing GO:0031123 IBA
    histone mRNA catabolic process GO:0071044 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified MTPAP in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MTPAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLIRP 81892
Proximity Label-MS Homo sapiens
3 HOOK1  
Proximity Label-MS Homo sapiens
4 STK25 10494
Affinity Capture-MS Homo sapiens
5 LAMP3  
Affinity Capture-MS Homo sapiens
6 GTF2IRD1  
Affinity Capture-MS Homo sapiens
7 TRIM66  
Affinity Capture-MS Homo sapiens
8 TRUB2  
Proximity Label-MS Homo sapiens
9 RPL10 6134
Affinity Capture-MS Homo sapiens
10 CALM1 801
Affinity Capture-MS Homo sapiens
11 CD79A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 MTRF1  
Proximity Label-MS Homo sapiens
14 LRPPRC 10128
Proximity Label-MS Homo sapiens
15 LRP1 4035
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 DIMT1 27292
Affinity Capture-MS Homo sapiens
17 EMILIN1 11117
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TFAM 7019
Proximity Label-MS Homo sapiens
19 FAM174A 345757
Affinity Capture-MS Homo sapiens
20 HEY1  
Affinity Capture-MS Homo sapiens
21 YARS2  
Affinity Capture-MS Homo sapiens
22 NPM1 4869
Affinity Capture-MS Homo sapiens
23 FBXW8 26259
Affinity Capture-MS Homo sapiens
24 CALCOCO2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 OBSL1 23363
Affinity Capture-MS Homo sapiens
26 NEDD8-MDP1 100528064
Affinity Capture-MS Homo sapiens
27 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ATG16L1 55054
Affinity Capture-MS Homo sapiens
29 FASTKD2  
Proximity Label-MS Homo sapiens
30 VWA8 23078
Proximity Label-MS Homo sapiens
31 MTERF3  
Proximity Label-MS Homo sapiens
32 DYNLT1 6993
Proximity Label-MS Homo sapiens
33 DMRTB1  
Two-hybrid Homo sapiens
34 NF2 4771
Affinity Capture-MS Homo sapiens
35 ATG4A  
Affinity Capture-MS Homo sapiens
36 C6orf203  
Proximity Label-MS Homo sapiens
37 FASTKD5  
Proximity Label-MS Homo sapiens
38 FOXI2  
Affinity Capture-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 MAPRE1 22919
Affinity Capture-MS Homo sapiens
41 VSIG4  
Affinity Capture-MS Homo sapiens
42 LRRC25  
Affinity Capture-MS Homo sapiens
43 AZI2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
44 PRKRA 8575
Affinity Capture-MS Homo sapiens
45 ZNF333  
Affinity Capture-MS Homo sapiens
46 GPR182  
Affinity Capture-MS Homo sapiens
47 THBS3 7059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 GFM1 85476
Proximity Label-MS Homo sapiens
49 HECTD1 25831
Affinity Capture-MS Homo sapiens
50 GEMIN7  
Affinity Capture-MS Homo sapiens
51 ARPC5 10092
Affinity Capture-MS Homo sapiens
52 MRPL11 65003
Proximity Label-MS Homo sapiens
53 MTRF1L  
Proximity Label-MS Homo sapiens
54 NCL 4691
Affinity Capture-MS Homo sapiens
55 FOXS1  
Affinity Capture-MS Homo sapiens
56 CALML3 810
Affinity Capture-MS Homo sapiens
57 RRP8  
Affinity Capture-MS Homo sapiens
58 MRRF  
Proximity Label-MS Homo sapiens
59 PRMT6  
Affinity Capture-MS Homo sapiens
60 MRPS12  
Proximity Label-MS Homo sapiens
61 MTIF2 4528
Proximity Label-MS Homo sapiens
62 LMNB1 4001
Proximity Label-MS Homo sapiens
63 MTIF3  
Proximity Label-MS Homo sapiens
64 SURF6  
Affinity Capture-MS Homo sapiens
65 MGARP  
Affinity Capture-MS Homo sapiens
66 CUL3 8452
Affinity Capture-MS Homo sapiens
67 EGFR 1956
Negative Genetic Homo sapiens
68 FAHD1 81889
Affinity Capture-MS Homo sapiens
69 HOOK3 84376
Proximity Label-MS Homo sapiens
70 PTK2 5747
Proximity Label-MS Homo sapiens
71 DHX30 22907
Proximity Label-MS Homo sapiens
72 LGALS7 3963
Affinity Capture-MS Homo sapiens
73 RIPK4  
Affinity Capture-MS Homo sapiens
74 SPACA1 81833
Affinity Capture-MS Homo sapiens
75 GRSF1 2926
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
76 C12orf65  
Proximity Label-MS Homo sapiens
77 NIN 51199
Proximity Label-MS Homo sapiens
78 DCAF8L2  
Affinity Capture-MS Homo sapiens
79 LGALS7B 653499
Affinity Capture-MS Homo sapiens
80 ACTL6B  
Affinity Capture-MS Homo sapiens
81 DDRGK1 65992
Affinity Capture-MS Homo sapiens
82 Dynll1 56455
Affinity Capture-MS Mus musculus
83 LGALS8 3964
Affinity Capture-MS Homo sapiens
84 PEX3 8504
Proximity Label-MS Homo sapiens
85 SOX2  
Affinity Capture-MS Homo sapiens
86 CLPP 8192
Proximity Label-MS Homo sapiens
87 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
88 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
89 CLK1  
Two-hybrid Homo sapiens
90 TSFM 10102
Proximity Label-MS Homo sapiens
91 TSPYL6  
Affinity Capture-MS Homo sapiens
92 ICT1 3396
Proximity Label-MS Homo sapiens
93 DDHD1 80821
Affinity Capture-MS Homo sapiens
94 PDHA1 5160
Proximity Label-MS Homo sapiens
95 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
96 COX8A  
Proximity Label-MS Homo sapiens
97 C21orf33  
Proximity Label-MS Homo sapiens
98 SSBP1 6742
Proximity Label-MS Homo sapiens
99 TBK1 29110
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
100 C10orf88  
Affinity Capture-MS Homo sapiens
101 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
102 TBRG4 9238
Proximity Label-MS Homo sapiens
103 AARS2  
Proximity Label-MS Homo sapiens
104 TEFM  
Proximity Label-MS Homo sapiens
105 BRD1 23774
Affinity Capture-MS Homo sapiens
106 EID1  
Affinity Capture-MS Homo sapiens
107 METTL17  
Proximity Label-MS Homo sapiens
108 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 TRAV20  
Affinity Capture-MS Homo sapiens
110 TRMU  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MTPAP is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here